Anti-Calcium Channel Antibody, Voltage Gated α 1C

Voltage-sensitive calcium channels (vscc) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1c gives rise to l-type calcium currents. Long-lasting (l-type) calcium channels belong to the "high-voltage activated" (hva) group. They are blocked by dihydropyridines (dhp), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-aga-IIIA). They are however insensitive to omega-conotoxin- gvia (omega-ctx-gvia) and omega-agatoxin-IVA (omega-aga-IVA). Calcium channels containing the alpha-1C subunit play an important role in excitation-contraction coupling in the heart. The various isoforms display marked differences in the sensitivity to dhp compounds (Swiss Prot).

Recognizes alpha 1C subunit (both LMW and HMW forms) of voltage-gated calcium channel. Does not cross react with any other calcium channel antigens tested so far. Guinea pig (17/18 residues identical); human and rabbit (16/18 residues identical).

Purified peptide (C)TTKI NMDDL QPSEN EDKS (CNC1) from 848-865 of alpha 1C subunit of rat brain voltage-gated calcium channel (Accession number P22002). Epitope location is in the intracellular loop between domains II and III.

Alpha 1 subunits of voltage-gated Ca2+ channels are highly sensitive to proteases. All procedures that are going to receive a full-length protein should be performed at 4°C, and the following protease inhibitor mixture should be used: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each).

Immunohistochemistry: 1:200 on rat brain sections.

Western blot: 1:200 using ECL on rat brain and heart material. LMW form shows a 190kDa band while the HMW form shows a 210kDa band.

Immunoprecipitation

Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions and protocols must be determined by the end user.

Detect Calcium Channel using this Anti-Calcium Channel Antibody, Voltage Gated α 1C, validated for use in IH, IP & WB.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Signaling Neuroscience

190 & 210 kDa

Affinity Purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

ImmunoAffinity Purified

Maintain lyophilized material at -20°C for up to 12 months. After reconstitution add glycerol (ASC grade or better) at a ratio of 1:1 and maintain at -20°C for up to 6 months. Avoid repeated freeze/thaw cycles.

Control
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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