Yeast Nitrogen Base With Amino Acids

Yeast Nitrogen Base is a highly-referenced growth medium used for the cultivation of yeast. This nutrient-rich microbial broth contains amino acids, nitrogen, vitamins, trace elements and salts.

Yeast Nitrogen Base With Amino Acids has been used:

• in the ZYP-31 media preparation for E.coli[1]
• in synthetic dextrose (SD) medium to evaluate the influence of nitrogen source[2]
• to culture Candida utilis for the production of ¹³C-labeled yeast extract as internal standard[3]
• in the preparation of imaging medium[4]

Suitable for use in classifying yeasts based on carbon requirements. Also suitable for general growth of yeasts with the addition of a carbon source (usually glucose).Yeast nitrogen base with amino acids is a satisfactory nitrogen source for general growth media. Contains all essential nutrients required for the growth of yeasts except carbohydrate.

Component, amount (μg/L unless indicated)

Nitrogen Sources:
Ammonium sulfate, 5.0 g/L

Amino Acids:
L-histidine, 10 mg/L
DL-methionine, 20 mg/L
DL-tryptophan, 20 mg/L

Vitamins:
Biotin, 2.0
Calcium pantothenate, 400
Folic acid, 2.0
Inositol, 2.0 mg/L
Nicotinic acid, 400
p-Aminobenzoic acid, 200
Pyridoxine HCl, 400
Riboflavin, 200
Thiamine HCL, 400

Trace Elements:
Boric acid
Copper sulfate
Potassium iodide
Ferric chloride
Magnesium sulfate
Sodium molybdate
Zinc sulfate

Salts:
Potassium phosphate monobasic, 1.0 g/L
Magnesium sulfate, 0.5 g/L
Sodium chloride, 0.1 g/L
Calcium chloride, 0.1 g/L

Make a 10x concentrated stock solution by stirring to suspend 6.7g yeast nitrogen base powder and 5 g glucose in 100 mL water. Warm if necessary to aid solubilization. Filter sterilize and store at 2-8°C.

Dilute the 10x concentrated stock to a 1x working solution by adding 100mL concentrated stock to 900mL sterile water.