Yeast Nitrogen Base Without Amino Acids

Yeast Nitrogen Base is a highly-referenced growth medium used for the cultivation of yeast. This nutrient-rich microbial broth contains nitrogen, vitamins, trace elements and salts.

Yeast Nitrogen Base is considered ideal for culturing wild type yeast. The presence of amino acid is known to prevent selectable marker utilization. Hence, this media is formulated without amino acids and is useful for cloning and manipulation of yeast artificial chromosome.

Suitable for use in classifying yeasts based on amino acid and carbon requirements. Addition of amino acids and carbon source (usually glucose) may be required.

Yeast Nitrogen Base Without Amino Acids has been used:

• in synthetic complete supplement mixture (SC)-Leu media used for Y. lipolytica culturing and characterization
• as a component of selective liquid media for uracil auxothrophic growth in yeast
• to evaluate the transformation efficiencies of S. cerevisiae strains, under different nutrient conditions

Component, amount (μg/L unless indicated)

Nitrogen Sources:
Ammonium sulfate, 5.0 g/L

Vitamins:
Biotin, 2.0
Calcium pantothenate, 400
Folic acid, 2.0
Inositol, 2.0 mg/L
Nicotinic acid, 400
p-Aminobenzoic acid, 200
Pyridoxine HCl, 400
Riboflavin, 200
Thiamine HCL, 400

Trace Elements:
Boric acid, 500
Copper sulfate, 40
Potassium iodide, 100
Ferric chloride, 200
Manganese sulfate, 400
Sodium molybdate, 200
Zinc sulfate, 400

Salts:
Potassium phosphate monobasic, 1.0 g/L
Magnesium sulfate, 0.5 g/L
Sodium chloride, 0.1 g/L
Calcium chloride, 0.1 g/L

1. Prepare a 10× stock solution by suspending 6.7 g of yeast nitrogen base, 5 g of glucose or an equivalent amount of other carbohydrate, and 5-10 mg of the desired amino acid in 100 ml of distilled water.
2. Warm if necessary to solubilize and sterilize by filtration.
Store 10× stock solution at 2-8 °C. To use, dilute 1:10 with sterile distilled water under aspetic conditions.