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Safety Information

TOX4

Sigma-Aldrich

In Vitro Toxicology Assay Kit, Neutral Red based

Synonym(s):

NR uptake assay, Neutral red assay

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1 KIT
¥4,117.37

¥4,117.37

Estimated to ship on2025年7月22日Details

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1 KIT
¥4,117.37

About This Item

UNSPSC Code:
12352207
NACRES:
NA.84

¥4,117.37

Estimated to ship on2025年7月22日Details

form

liquid

Quality Level

200

usage

 kit sufficient for 1,000 tests

packaging

pkg of 1 kit

storage condition

dry at room temperature

λmax

540 nm

application(s)

cell analysis
detection

detection method

colorimetric

storage temp.

2-8°C

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体外毒性检测试剂盒，基于乳酸脱氢酶

TOX7

体外毒性检测试剂盒，基于乳酸脱氢酶

usage

 kit sufficient for 1,000 tests

usage

 kit sufficient for 2,000 tests

usage

 kit sufficient for 1,000 tests

usage

 kit sufficient for 500 tests

packaging

pkg of 1 kit

packaging

pkg of 1 kit

packaging

pkg of 1 kit

packaging

pkg of 1 kit

storage condition

dry at room temperature

storage condition

dry at room temperature

storage condition

dry at room temperature

storage condition

dry at room temperature

λmax

540 nm

λmax

-

λmax

570 nm

λmax

490 nm

cytotoxicity assay substrate, colorimetric determination, 540 nM detection

cytotoxicity assay substrate, tetrazolium dye based, fluroescence determination, 600 nM detection

cytotoxicity assay substrate, MTT dye based, colorimetric determination, 570 nM detection

cytotoxicity assay substrate, tetrazolium dye based, colorimetric determination, 490 nM detection

General description

For spectrophotometric measurement of viable cells. Absorbance of converted dye is measured at a wavelength of 540 nm.

Application

In Vitro Toxicology Assay Kit, Neutral Red based has been used:
  • to measure the lysosomal enzyme activity in HB8902 cells[1]
  • to study the cell viability of human alveolar epithelial cells[2]
  • to study the cell viability in fibroblasts[3]

Biochem/physiol Actions

Neutral red (NR) is a red dye that accumulates in the lysosome by actively transferring across the cell membranes.[3] The dye is extracted and the uptake is quantitated by spectroscopy. The NR uptake assay is the most commonly used cytotoxicity test. It is widely applicable in environmental and biomedical studies to estimate the number of viable cells in culture. The NR dye in vitro tissue culture studies is used in assessing viral cytopathogenicity and detection of toxic compounds.[4]

Signal Word

Danger

Hazard Statements

H226,H317,H350

Hazard Classifications

Carc. 1B - Flam. Liq. 3 - Skin Sens. 1

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

73.4 °F

Flash Point(C)

23 °C

Regulatory Information

危险化学品
  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    0000344662
    0000344662
    SLCP8359
    SLCL3641
    SLCK0277

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    H Babich et al.
    Ecotoxicology and environmental safety, 21(3), 327-336 (1991-06-01)
    The PLHC-1 fish hepatoma cell line (Poeciliopsis lucida) was used in the neutral red assay to evaluate the acute cytotoxicities of direct-acting (alkylbenzenes, phthalate diesters, and pesticides) and metabolism-mediated (benzo[a]pyrene) toxicants. The sequence of cytotoxic potencies for the alkylbenzenes and
    Simonetta Palleschi et al.
    Chemosphere, 196, 35-44 (2018-01-01)
    Fine airborne particulate matter (PM2.5) has been repeatedly associated with adverse health effects in humans. The PM2.5 soluble fraction, and soluble metals in particular, are thought to cause lung damage. Literature data, however, are not consistent and the role of
    Adam L Nathan et al.
    Journal of medical devices, 11(1), 0110091-110099 (2017-02-10)
    Highly porous, open-celled shape memory polymer (SMP) foams are being developed for a number of vascular occlusion devices. Applications include abdominal aortic and neurovascular aneurysm or peripheral vascular occlusion. A major concern with implanting these high surface area materials in
    Thorsten M Seyler et al.
    Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association, 33(2), 374-386 (2016-10-04)
    To evaluate the biological, immunological, and biomechanical properties of a scaffold derived by architectural modification of a fresh-frozen porcine patella tendon using a decellularization protocol that combines physical, chemical, and enzymatic modalities. Porcine patellar tendons were processed using a decellularization
    Guillermo Repetto et al.
    Nature protocols, 3(7), 1125-1131 (2008-07-05)
    The neutral red uptake assay provides a quantitative estimation of the number of viable cells in a culture. It is one of the most used cytotoxicity tests with many biomedical and environmental applications. It is based on the ability of
    View All Related Papers

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