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TOX6

Sigma-Aldrich

体外毒理学检测试剂盒，基于磺酰罗丹明B

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别名:
biomass viability assay, sulforhodamine B, total biomass assay
UNSPSC代码:
12352207
NACRES:
NA.84

形式

liquid

质量水平

200

用途

 kit sufficient for 1,000 tests

包装

pkg of 1 kit

储存条件

dry at room temperature

λmax

565 nm

应用

cell analysis
detection

检测方法

colorimetric

储存温度

room temp

一般描述

磺酰罗丹明B方法简便、准确，结果一致。该分析系统是一种测量总生物量的方法，通过染色细胞蛋白与硫血红蛋白B。

应用

基于磺酰罗丹明B的体外毒性检测试剂盒已被用于通过总蛋白进行生物质（活细胞和死细胞）的分光光度测定。它用于研究17-烯丙基氨基-17-去甲氧格尔德霉素 (17-AAG) 对五种葡萄膜黑色素瘤细胞系增生的作用。在565nm波长处测量染料的吸光度。

生化/生理作用

染料与细胞蛋白结合并随后溶解在碱中。

警示用语：

Danger

危险声明

H226,H314,H410

危险分类

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Skin Corr. 1A

WGK

WGK 2

法规信息

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. When using Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B based, what happens if I add kit component Product T5413, 10 mM Tris Base Solution, prior to fixing the cells?

    If the 10 mM Tris Base Solution, Product T5413 is added to the cells before the TCA (fixative), the cells will be lysed.  This reagent is not buffered and is not isotonic. The assay cannot be rescued if this occurs.

  6. Can you use Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B based with suspension cells?

    Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B can be used with both adherent and suspension culture.The advantage of layering the fixative directly onto the media is that this allows the assay to work for cells in suspension.  Otherwise, you need to centrifuge, remove the media and then fix.  Also, it is possible to loose cells when you remove the media. It was found that adding the fixative works for the assay and the protocol was changed in 2003. 

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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