推荐产品
表单
liquid
质量水平
用途
1 kit sufficient for 1,000 tests
包装
pkg of 1 kit
储存条件
dry at room temperature
λmax
540 nm
应用
cell analysis
detection
检测方法
colorimetric
储存温度
2-8°C
一般描述
用于分光光度法活细胞测定。在540 nm波长下检测转化染料的吸光度。
应用
基于中性红的体外毒理检测试剂盒已用于：
- 测定HB8902细胞内溶酶体酶活性
- 研究人肺泡上皮细胞的细胞活力
- 研究成纤维细胞的细胞活力
生化/生理作用
中性红(NR)是一种红色染料，通过细胞膜主动转移在溶酶体中积累。染料被提取并根据光谱定量吸收。NR摄取试验是最常用的细胞毒性试验。它广泛应用于环境和生物医学研究中，用于估计培养中的活细胞数量。NR染料体外组织培养研究用于评估病毒细胞致病性和检测有毒化合物。
警示用语：
Danger
危险声明
危险分类
Carc. 1B - Flam. Liq. 3 - Skin Sens. 1
储存分类代码
3 - Flammable liquids
WGK
WGK 3
闪点(°F)
73.4 °F
闪点(°C)
23 °C
法规信息
危险化学品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
D Triglia et al.
In vitro cellular & developmental biology : journal of the Tissue Culture Association, 27A(3 Pt 1), 239-244 (1991-03-01)
A new three-dimensional human skin model consisting of several layers of actively dividing and metabolically active human neonatal foreskin-derived fibroblasts and epidermal keratinocytes grown on nylon mesh has been used to assess the in vitro toxicity of test agents from
Guillermo Repetto et al.
Nature protocols, 3(7), 1125-1131 (2008-07-05)
The neutral red uptake assay provides a quantitative estimation of the number of viable cells in a culture. It is one of the most used cytotoxicity tests with many biomedical and environmental applications. It is based on the ability of
Barbara Kazuń et al.
BMC veterinary research, 16(1), 216-216 (2020-06-27)
The aim of the study has been to compare the effect of dietary supplementation of β-1,3/1,6-glucan, Lactobacillus plantarum bacteria or their mixture on the growth performance, selected parameters of the immune system as well as the liver and intestinal histology
Elisabetta Chieli et al.
Chemico-biological interactions, 186(3), 287-294 (2010-06-02)
Many plant compounds are able to modulate the activity and/or the expression of the major multidrug transporter ABCB1/P-glycoprotein (P-gp). In this study, mango (Mangifera indica L.) stem bark extract (MSBE), its main polyphenol mangiferin and the mangiferin aglycone derivative norathyriol
E Borenfreund et al.
Toxicology letters, 24(2-3), 119-124 (1985-02-01)
A method is described which combines the use of a visual morphological cytotoxicity assay with a quantitative neutral red (NR) spectrophotometric test, for the assessment of the effect of toxic agents on 3T3 cells in culture. These sensitive and reproducible
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门