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TOX1

Sigma-Aldrich

体外毒理学检测试剂盒（基于MTT法）

别名:

mitochondrial activity assay

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About This Item

UNSPSC代码:
12352207
NACRES:
NA.84

用途

 kit sufficient for 1,000 tests

质量水平

200

包装

pkg of 1 kit

储存条件

dry at room temperature

λmax

570 nm

应用

cell analysis
detection

检测方法

colorimetric

储存温度

2-8°C

相关类别

一般描述

本试剂盒旨在利用活细胞的线粒体活性功能通过比色法测定细胞数/细胞活力。MTT法是一种简便、准确、结果可重复的方法。关键组分是(3-[4,5-二甲基-2-噻唑基]-2,5-二苯基溴化四唑) 或MTT。溶于无酚红的培养基或平衡盐溶液中的MTT溶液呈浅黄色。其四唑环可被活细胞的线粒体脱氢酶剪切而产生不溶于水溶液的紫色甲臜晶体。在570nm波长处测量转化染料的吸光度。

应用

MTT体外细胞毒性检测试剂盒已用于通过(3-[4,5-二甲基-2-噻唑基]-2,5-二苯基溴化四唑)MTT法检测金纳米颗粒(AuNP)的细胞毒性。它还用于检测SH-SY5Y培养物经过6-羟基多巴胺(6-OHDA)处理后的细胞活性。

生化/生理作用

将MTT转化成水不溶性的有色甲臜衍生物，然后用酸性异丙醇溶解。

警示用语：

Danger

危险分类

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Skin Corr. 1 - STOT SE 3

靶器官

Central nervous system, Respiratory system

储存分类代码

3 - Flammable liquids

闪点(°F)

53.6 °F

闪点(°C)

12 °C

法规信息

危险化学品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Is the MTT assay quantitative?

    The MTT asay is a method to assess cell viability.  This assay is a semiquantitative assay. The assay is used to compare the viability changes in treated cells to untreated cells. The absorbance is indicative of the cell number. The higher the absorbance, the greater the number of viable cells present. Most researchers compare the absorbance of the two samples as a ratio (ABS treated cells/ABS untreated cells) to get a fold increase/decrease in cell number.   

  6. What is the difference between product CGD1, Cell Growth Determination Kit and product TOX1, In Vitro Toxicology Assay Kit?

    Product CGD1 and TOX1 are both MTT based assays. The major difference between product CGD1 and TOX1 is that TOX1 contains 10 % Triton X-100 in the solubilization solution for better lysis of the cells and solubilization of the MTT.  If there is complete cell lysis, the results obtained with both kits would be the same.  The MTT in this kit is a solution, whereas the TOX1 assay kit contains the MTT as a powder that is solubilized before use.

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

J Carmichael et al.
Cancer research, 47(4), 936-942 (1987-02-15)
Drug sensitivity assays were performed using a variation of a colorimetric [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)] assay on V79, CHO-AuxB1, CHRC5, NCI-H460, and NCI-H249 cell lines following optimization of experimental conditions for each cell line. Results from this assay were compared with
Dalia Chávez-García et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 108(6), 2396-2406 (2020-02-06)
Luminescent lanthanide downconversion nanoparticles (DCNPs) provide a combination of high luminescence intensity, sharp emission peaks with narrow bandwidth and a large Stokes' shift, leading to high-performance biomedical applications mainly for imaging. The purpose of this study is to present a
Srijayaprakash B Uppada et al.
International journal of nanomedicine, 9, 43-53 (2014-01-07)
Oligonucleotides homologous to 3'-telomere overhang (T-oligos) trigger inherent telomere-based DNA damage responses mediated by p53 and/or ATM and induce senescence or apoptosis in various cancerous cells. However, T-oligo has limited stability in vivo due to serum and intracellular nucleases. To
Anumol Shashikumar et al.
Cytotechnology, 63(5), 473-480 (2011-07-30)
This is the first report on development of a finite cell line from testicular tissues of crab, Scylla serrata. Both the explant and segregated tissues of testes yielded cells that could proliferate and grow. These cells ranged in size from
F Denizot et al.
Journal of immunological methods, 89(2), 271-277 (1986-05-22)
A convenient way to estimate the number of viable cells growing in microtitre tray wells is to use a colorimetric assay and an automatic microplate scanning spectrophotometer. One such assay, developed by Mosmann, depends on the reduction by living cells
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