R4775
REDExtract-N-Amp™ PCR ReadyMix™
Ready-to-use 2X PCR Master Mix with Loading Dye
Recommended Products
Quality Level
usage
sufficient for 100 reactions
1.2 mL sufficient for 100 amplifications
sufficient for 1000 reactions
12 mL sufficient for 1000 amplifications
sufficient for 10000 reactions
125 mL sufficient for 10000 amplifications
feature
dNTPs included
hotstart
color
red
application(s)
agriculture
shipped in
wet ice
storage temp.
−20°C
Related Categories
General description
This PCR ReadyMix is intended for use with Sigma′s Extract-N-Amp Plant PCR kit and Extract-N-Amp Tissue PCR Kit. All Extract-N-Amp kits are sufficient for one PCR amplification per extraction. However, where additional PCR reactions are required, this supplemental PCR ReadyMix may be needed. The REDExtract-N-Amp PCR ReadyMix contains an inert tracking dye that allows for convenient direct loading of PCR products onto agarose gels for analysis.
Application
- for the genotyping of fixed zebrafish embryo samples
- in polymerase chain reaction (PCR) amplification of the DNA isolated from lichen family using the REDExtract-N-Amp™ Plant PCR Kit
- in the amplification of DNA isolated from single yeast colonies and fungal spores using REDExtract-N-Amp™ seed PCR kit.
REDExtract-N-Amp™ PCR Master Mix is also suitable for colony PCR.
Features and Benefits
- A Gel-ready- No loading buffer or tracking dye needs to be added. The PCR product is loaded directly into an agarose gel after amplification. The red tracer migrates at approximately the same rate as a 125bp fragment (slightly faster than bromophenol blue).
- Convenience- Hot-start feature in JumpStart™ products allows reaction set up at room temperature, which can be beneficial when working with multiple samples. Ready-mix format reduces setup time.
- Reproducibility- Reduces the risk of contamination from multiple pipet steps and provides consistent performance across both reactions and time.
- High Specificity & Yield- JumpStart Taq DNA polymerase, an antibody inactivated hot-start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity.
Legal Information
also commonly purchased with this product
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Extract-N-Amp™ Tissue PCR Kit quickly extracts PCR-ready DNA from mouse tails, providing optimized PCR mix for efficient amplification.
Colony PCR reagents and our colony PCR protocol using REDExtract-N-Amp™ PCR ReadyMix™ and JumpStart™ REDTaq® PCR ReadyMix™ reagents.
Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.
Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.
Protocols
REDExtract-N-Amp Plant PCR kit includes loading dye for easy gel loading after plant genomic DNA extraction.
Extract-N-Amp kit protocol provides rapid DNA extraction yielding PCR-ready samples in 15 minutes with optimized PCR ReadyMix.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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