Extract-N-Amp^™ Plant PCR Kit

The Extract-N-Amp Plant PCR Kits contain all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. A novel Extraction Solution eliminates the need for conventional freezing of plant tissues with liquid nitrogen, mechanical disruption, organic extraction, column purification, or precipitation of DNA. The kit also includes a PCR ReadyMix^™, especially formulated for amplification directly from extract.

Genomic DNA is extracted from 0.5 to 0.7 cm plant leaf disks that have been cut with a standard paper punch and simply incubated in Extraction Solution at 95 ^oC for 10 minutes. An equal volume of Dilution Solution is added to the extract to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and the Extract-N-Amp PCR ReadyMix, included in the kit.

The Extract-N-Amp^™ Plant PCR Kits are intended for direct PCR (polymerase chain reaction). This kit contains all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. It is a novel Extraction Solution that eliminates the need for conventional freezing of plant tissues.

Extract-N-Amp^™ Plant PCR Kit has been used for genomic DNA isolation from plants and the fungal mycelium. It has also been used in polymerase chain reaction (PCR).

Extract-N-Amp^™ Plant PCR Kit is suitable for use in genotyping.

It has been used forDNA extraction from fresh mushrooms.

Extract-N-Amp^™ Plant PCR Kits contain Dilution solution, Extraction solution, and Extract-N-Amp PCR Reaction Mix (2X) (buffer, salts, dNTPs, Taq polymerase, and JumpStart Taq antibody)

For additional information, please see www.sigma-aldrich.com/extract-n-amp.

The Extract-N-Amp Plant PCR Kits are for laboratory use only. Not for drug, household, or other uses.

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC