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I9516

Sigma-Aldrich

2-Propanol

≥99.5%, for molecular biology, suitable for RNA extraction, DNA extraction, BioReagent

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Synonym(s):
sec-Propyl alcohol, IPA, Isopropanol, Isopropyl alcohol
Linear Formula:
(CH3)2CHOH
CAS Number:
67-63-0
Molecular Weight:
60.10
Beilstein:
635639
EC Number:
200-661-7
MDL number:
MFCD00011674
UNSPSC Code:
12352104
PubChem Substance ID:
24896158
NACRES:
NA.52

grade

for molecular biology

Quality Level

200

vapor density

2.1 (vs air)

vapor pressure

33 mmHg ( 20 °C)
44 mmHg ( 25 °C)

product line

BioReagent

Assay

≥99.5%

form

liquid

autoignition temp.

750 °F

expl. lim.

2.0-12.7 %, 93 °C

technique(s)

DNA extraction: suitable
RNA extraction: suitable

impurities

≤0.2% water
water

color

colorless

refractive index

n20/D 1.377 (lit.)

bp

82 °C (lit.)

mp

−89.5 °C (lit.)

solubility

water: soluble (completely)

density

0.785 g/mL at 25 °C (lit.)

suitability

suitable for precipitation of nucleic acids

storage temp.

room temp

SMILES string

CC(C)O

InChI

1S/C3H8O/c1-3(2)4/h3-4H,1-2H3

InChI key

KFZMGEQAYNKOFK-UHFFFAOYSA-N

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General description

Isopropanol is a clear, colorless, polar organic solvent commonly used in chemistry and molecular biology laboratories. It will dissolve a wide range of chemicals, and evaporates quickly. This product (2-propanol) is designated as molecular biology grade and is suitable for the precipitation of nucleic acids.

Protocols for the use of isopropanol:

  • DNA precipitation from mammalian tissue, ethidium bromide extraction from DNA samples, and RNA isolation from cells and tissues.
  • Protein crystallization.
  • Isolate retinoids from biological matrices for HPLC and HPLC-MS analysis.

Application

2-Propanol has been used:
  • in DNA and RNA extraction
  • for determination of hydroxyproline levels(The Merck Index, 12th ed)
  • for checking cell viability by MTT assay
  • in microarray hybridization
Suitable for use in DNA precipitation using standard protocols. Also suitable as a solvent for making solutions for molecular biology applications.

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Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H225,H319,H336

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

Target Organs

Central nervous system

WGK

WGK 1

Flash Point(F)

53.6 °F

Flash Point(C)

12.0 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

危险化学品

Certificates of Analysis (COA)

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S Knapp et al.
Proteins, 23(4), 595-597 (1995-12-01)
The thermostable amylopullulanase from Pyrococcus woesei was crystallized. Crystals, suitable for a crystallographic analysis up to a size of 0.6 mm in their longest dimension, have been obtained by the vapor diffusion method in a solution containing polyethyleneglycol 4000 (PEG
Robert B Nicewonger et al.
Bioorganic & medicinal chemistry letters, 12(14), 1799-1802 (2002-06-28)
In this paper, we describe the synthesis of N-(6-cyano-1,3-dimethyl-2,4-dioxo-5-substituted-1,3-dihydropyridino[2,3-d] pyrimidin-7-yl)imides 1. We will show the synthesis of 1 using both conventional heating and microwave techniques. In addition, the imide was attached to polystyrene and this immobilized imide was equally as
M J Romão et al.
European journal of biochemistry, 215(3), 729-732 (1993-08-01)
The Desulfovibrio gigas aldehyde oxidoreductase contains molybdenum bound to a pterin cofactor and [2Fe-2S] centers. The enzyme was characterized by SDS/PAGE, gel-filtration and analytical ultracentrifugation experiments. It was crystallized at 4 degrees C, pH 7.2, using isopropanol and MgCl2 as
Christudas Morais et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 25(5), 1462-1474 (2009-12-29)
Renal cell carcinoma (RCC) is a highly metastatic and lethal disease with few efficacious treatments. Many studies have shown that the ubiquitous transcription factor nuclear factor kappa B (NF-kappaB) plays a key role in the development and progression of many
Andrew S Welleford et al.
Cell transplantation, 29, 963689720926157-963689720926157 (2020-05-20)
The development of regenerative therapies for central nervous system diseases can likely benefit from an understanding of the peripheral nervous system repair process, particularly in identifying potential gene pathways involved in human nerve repair. This study employed RNA sequencing (RNA-seq)
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