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P5368

Sigma-Aldrich

Phosphate buffered saline

powder, pH 7.4, BioPerformance Certified, for molecular biology

Synonym(s):

PBS

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About This Item

MDL number:
MFCD00131855
UNSPSC Code:
12161700
eCl@ss:
32129211
NACRES:
NA.25

product name

Phosphate buffered saline, BioPerformance Certified, pH 7.4

grade

BioPerformance Certified
for molecular biology

Quality Level

200

form

powder

storage condition

(Keep container tightly closed in a dry and well-ventilated place.)

technique(s)

immunofluorescence: suitable

impurities

BioBurden, tested
 endotoxin, tested

color

white

pH

7.4

solubility

water: 9.07-10.0 g/L, clear, colorless

cation traces

Fe: ≤10 ppm
heavy metals (as Pb): ≤5 ppm

suitability

suitable for SDS-PAGE
suitable for molecular biology

application(s)

diagnostic assay manufacturing
histology
sample preparation

foreign activity

DNase, RNase, Protease, none detected

SMILES string

OP(=O)(O)[O-].OP(=O)([O-])[O-].[Na+].[Na+].[Na+].[Cl-].[Cl-].[K+].[K+]

InChI

InChI=1S/2ClH.2K.3Na.2H3O4P/c;;;;;;;2*1-5(2,3)4/h2*1H;;;;;;2*(H3,1,2,3,4)/q;;5*+1;;/p-5

InChI key

LOKCTEFSRHRXRJ-UHFFFAOYSA-I

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Application


  • Rapid and zero-cost DNA extraction from soft-bodied insects for routine PCR-based applications.: This study used phosphate buffered saline (PBS) to develop a cost-effective and efficient method for DNA extraction from insects, facilitating routine PCR-based applications in molecular biology (Jangra & Ghosh, 2022).

  • Injectable amnion hydrogel-mediated delivery of adipose-derived stem cells for osteoarthritis treatment.: PBS was utilized to prepare and deliver adipose-derived stem cells in an injectable hydrogel, showing potential for innovative osteoarthritis treatments (Bhattacharjee et al., 2022).

  • Rapid and sensitive detection of SARS-CoV-2 infection using quantitative peptide enrichment LC-MS analysis.: This research employed PBS for sample preparation in a novel LC-MS method for detecting SARS-CoV-2, providing a rapid and sensitive diagnostic tool (Hober et al., 2021).

Packaging

Foil Pouch

Reconstitution

Contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.01 M phosphate buffered saline (NaCl 0.138 M; KCl - 0.0027 M); pH 7.4, at 25 °C.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Does Product P5368, Phosphate buffered saline (PBS), contain calcium and magnesium?

    No.  The complete formulation is shown in the product listing.  When reconstituted, the resulting solution is 10 mM phosphate buffer, with 138 mM sodium chloride and 2.7 mM potassium chloride.

  4. If reconstituted with sterile water, will Product P5368, Phosphate buffered saline (PBS), be sterile?

    No.  To make this PBS sterile, the solution should be either sterile-filtered or autoclaved.

  5. What volume of Product P5368, Phosphate buffered saline (PBS), does each pack make?

    When the contents of a pouch is dissolved in 1 Liter of water, it makes 1X PBS, pH 7.4 at 25°C.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Jesús Gilberto Arámburo-Galvez et al.
Nutrients, 10(7) (2018-07-18)
Most food allergy cases are associated with a limited group of allergens. This could be attributed to an increased ability of some foods to sensitize and trigger allergic reactions. However, there are no validated animal models to evaluate the sensitizing
Petra J Roubos-van den Hil et al.
Veterinary microbiology, 202, 58-63 (2016-09-27)
Enterotoxigenic E. coli (ETEC), causing post-weaning diarrhoea, is a major problem in weaned piglets. Individual animal responses to ETEC infection show high variability in animal experiments. Two studies were designed to optimize the ETEC F4ac infection model in piglets by
Jinjin Sun et al.
Nanoscale research letters, 14(1), 248-248 (2019-07-26)
Herein, a magnetic graphene field-effect transistor biosensor was prepared through the transfer of a chemical vapor deposition graphene film onto a glass substrate to produce a sensing film and conductive channel. By fixing 1-pyrenebutanoic acid succinimidyl ester onto graphene film
Yixi Chen et al.
The European journal of neuroscience, 49(4), 510-524 (2018-11-26)
An emerging treatment for Parkinson's disease (PD) is cell replacement therapy. Authentic midbrain dopaminergic (mDA) neuronal precursors can be differentiated from human embryonic stem cells (hESCs) and human induced pluripotent stem cells (iPSCs). These laboratory-generated mDA cells have been demonstrated
Gary Burness et al.
Biology open, 7(4) (2018-04-06)
Female birds transfer maternally derived antibodies (matAb) to their nestlings, via the egg yolk. These antibodies are thought to provide passive protection, and allow nestlings to avoid the costs associated with mounting an innate immune response. To test whether there
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