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About This Item
UNSPSC Code:
12352211
NACRES:
NA.83
Product Name
Fluorescent Transfection Reagent, Avanti Research™ - A Croda Brand, powder
assay
>99% (TLC)
form
powder
packaging
pkg of 1 × 10 mg (790326P-10mg)
technique(s)
transfection: suitable
lipid type
transfection
cationic lipids
shipped in
dry ice
storage temp.
−20°C
manufacturer/tradename
Avanti Research™ - A Croda Brand
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Biochem/physiol Actions
The DOTAP:DOPE with 0.1% NBD-DOPE liposome (LipNBD) reconstituted with transferrin called the transferrin liposome-nitrobenzoxadiazole (Tf-LipNBD) is useful as a probe. It provides improved image contrast in in vitro magnetic resonance imaging (MRI) of tumor cells. Fluorescent transfection reagent aids in understanding fibroplex membrane structure and fibroin interaction with cationic lipids. N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)dioleoyl (NBD) is used in model membranes studies and is useful in visualizing membrane domains.
General description
Fluorescent transfection reagent has the cationic lipid 1,2-dioleoyloxypropyl)-N,N,N-trimethylammonium chloride (DOTAP) and the neutral lipid DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine in the ratio 1:1 w/w along with 0.1 wt% N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)dioleoyl (NBD) DOPE. NBD-DOPE is a fluorescence probe with NBD attached to headgroup of DOPE.
Packaging
5 mL Clear Glass Sealed Ampule (790326P-10mg)
Legal Information
Avanti Research is a trademark of Avanti Polar Lipids, LLC
Application
Transfection research
Storage Class
11 - Combustible Solids
flash_point_f
No data available
flash_point_c
No data available
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The influence of NBD fluorescent probe on model membranes containing POPC and DPPC
Weng CJ, et al.
Molecular Membrane Biology, 33(1-2), 23-28 (2016)
Efficiency data of intracellular recombinant protein delivery using cationic lipid coated silk fibroin particle
Kim WJ, et al.
Data in Brief, 13, 683-691 (2017)
Dual probe with fluorescent and magnetic properties for imaging solid tumor xenografts
Shan L, et al.
Molecular Imaging, 6(2), 7290-2007 (2007)
Woo-Jin Kim et al.
Biomaterials, 122, 154-162 (2017-01-26)
Directly delivering therapeutic proteins into cells has promise as an intervention without side effects for protein deficiencies caused by genetic defects. However, as negatively charged macromolecules, proteins require carriers for achieving cellular uptake and maintaining their activity in the cytoplasm.
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