Skip to Content

Dear Customer:

The current international situation is complex and volatile, and uncertain tariff policies may potentially impact our product prices. Given these uncertainties, we value your understanding regarding order-related matters.

If you decide to place an order during this period, we reserve the right to adjust the price based on the evolving situation. We understand that market changes may cause inconvenience. We will negotiate with you if there’s a significant price fluctuation due to tariff policy changes before the order’s actual delivery, and in such cases we may adjust or cancel the order as necessary.

Merck
CN
All Photos(1)

Key Documents

View All Documentation

Safety Information

P2602

SAFC

Peripheral Blood Medium

liquid, application tested

Sign Into View Organizational & Contract Pricing

Select a Size

50 G
CN¥6,984.41
100 G
CN¥12,349.49
6 X 100 G
CN¥50,600.71

CN¥6,984.41

Available to ship onApril 27, 2025Details

All Photos(1)

Select a Size

Change View
50 G
CN¥6,984.41
100 G
CN¥12,349.49
6 X 100 G
CN¥50,600.71

About This Item

UNSPSC Code:
12352207

CN¥6,984.41

Available to ship onApril 27, 2025Details

sterility

sterile-filtered

form

liquid

IVD

for in vitro diagnostic use

impurities

endotoxin, tested

suitability

application tested

shipped in

dry ice

storage temp.

−20°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
G50150G100120U8878
Sephadex® LH-20

LH20100

Sephadex® LH-20

Sephadex® G-50 Medium

G50150

Sephadex® G-50

Sephadex® G-100 Medium

G100120

Sephadex® G-100

Sigma-Aldrich

U8878

Ultrogel® AcA 34

matrix

Agarose

matrix

Agarose

matrix

Agarose

matrix

3% acrylamide, 4% agarose

separation technique

size exclusion (SEC)

separation technique

size exclusion (SEC)

separation technique

size exclusion (SEC)

separation technique

size exclusion (SEC)

matrix active group

polymer

matrix active group

polymer

matrix active group

polymer

matrix active group

polymer

form

slurry

form

slurry

form

slurry

form

aqueous ethanol suspension

technique(s)

LPLC: suitable

technique(s)

LPLC: suitable

technique(s)

LPLC: suitable

technique(s)

LPLC: suitable

storage temp.

room temp

storage temp.

-

storage temp.

-

storage temp.

2-8°C

Application

Peripheral Blood Medium has been developed for in vitro short-term growth of peripheral blood lymphocytes for chromosome analysis.

Peripheral Blood Medium supports mitotic indexes comparable or superior to the best available media. The quality of metaphases and improved chromosome morphology facilitates successful case completion. Because optimized lots of serum and PHA are utilized for production, fluctuation in product quality during manufacturing is minimized. The medium is offered as a complete, ready-to-use frozen solution requiring no supplementation. To ensure optimum results, an insert is provided with each package detailing product specifications and instructions for use.

Features and Benefits

Development of Sigma′s Peripheral Blood Medium involved extensive R&D work and parallel evaluations in a licensed cytogenetics laboratory. Initial efforts focused on the basal medium formulation with incorporation of components that improved the mitotic index. Subsequent efforts concentrated on the selection of serum and phytohemagglutin (PHA) lots by monitoring mitogenic response in a flow cytometer. Short-term (24 to 72 hours) cultures of human blood specimens were used for all assays. Medium samples were compared to several commercially available and custom-made media routinely used in cytogenetic laboratories. Analyses included chromosome morphology, spreading and band resolution level. Stability studies at various temperatures were used to evaluate medium performance and ensure the best storage temperature.

Other Notes

Proprietary formulation containing RPMI-1640 with L-glutamine, HEPES and sodium bicarbonate buffers, fetal bovine serum, phytohemagglutinin (PHA), and gentamicin sulfate.

Regulatory Information

新产品

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    092K2331
    063K2326
    032K2326
    023K2334
    012K2363

    Don't see the Right Version?

    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

    Search for a COA

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    M R O'Donovan et al.
    Mutagenesis, 10(4), 371-374 (1995-07-01)
    Although lymphocytes in phytohaemagglutinin (PHA)-stimulated whole blood cultures are routinely used to assess genotoxin-induced chromosome damage, very little information is available on the effect of PHA on the various cell populations present, and there appear to be no data for

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service