All Photos(1)
Key Documents
Safety Information
P0762
Phosphatase, Alkaline−Agarose from calf intestine
ammonium sulfate suspension
Synonym(s):
Alkaline phosphatase-Agarose
Sign Into View Organizational & Contract Pricing
Select a Size
50 UNITS
CN¥2,203.77
100 UNITS
CN¥4,468.64
250 UNITS
CN¥12,480.23
1000 UNITS
CN¥29,951.63
CN¥2,203.77
List PriceCN¥2,592.67Save 15%Please contact Customer Service for Availability
All Photos(1)
Select a Size
Change View
50 UNITS
CN¥2,203.77
100 UNITS
CN¥4,468.64
250 UNITS
CN¥12,480.23
1000 UNITS
CN¥29,951.63
About This Item
CN¥2,203.77
List PriceCN¥2,592.67Save 15%Please contact Customer Service for Availability
Recommended Products
![Guanosine 5′-[β,γ-imido]triphosphate trisodium salt hydrate ≥85% (HPLC), powder](/deepweb/assets/sigmaaldrich/product/structures/204/494/05808804-1ca7-44bf-b6c5-d4934dc7cb85/640/05808804-1ca7-44bf-b6c5-d4934dc7cb85.png)
biological source
bovine (calf) intestine
Quality Level
form
ammonium sulfate suspension
specific activity
250-1000 units/mL packed gel
matrix
beaded agarose
storage temp.
2-8°C
Related Categories
1 of 4
This Item | P6774 | P0114 | P7923 |
|---|---|---|---|
| specific activity 250-1000 units/mL packed gel | specific activity ≥2,000 DEA units/mg protein | specific activity ≥5,700 DEA units/mg protein | specific activity ≥4,000 DEA units/mg protein |
| biological source bovine (calf) intestine | biological source bovine intestinal mucosa | biological source - | biological source - |
| form ammonium sulfate suspension | form buffered aqueous solution | form (40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6) | form buffered aqueous glycerol solution |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| matrix beaded agarose | matrix - | matrix - | matrix - |
General description
Alkaline phosphatase from calf intestine is a glycoprotein with a molecular weight of 140,000. [1]
Application
Alkaline phosphatase from calf intestine, CIP, removes phosphate groups from the 5′ ends of DNA which aids in cloning techniques to keep DNA from self-ligation. It can be inhibited by homoarginine. [2]
Calf intestinal alkaline phosphatase (CIAP) has been used in a study with the spectrophotometric method to assess the inhibition effect of dansyl-L-phenylalanine. [3] It has also been shown that (CIAP) is able to detoxify the lipopolysaccharide (LPS)-mediated inflammatory response, which could be important in the treatment of LPS-mediated diseases. [4]
Biochem/physiol Actions
Sigma insoluble enzymes are produced by reacting the conventional "soluble" enzyme with an inert base that results in an insoluble compound retaining the activity of the original enzyme. Applications are almost unlimited. By selecting the "inert" base carefully, a highly active "resin" or "gel" can be produced with which the enzyme reaction can be quickly catalyzed by momentary contact with the substrate in a suitable medium. This can be carried out either in a batch slurry or even through a small column. In either case, the insoluble enzyme does not remain as a contaminant of the reaction mix. It can be readily filtered or centrifuged out of the batch slurry, or remain behind in the column. Many unstable enzymes seem to be much more stable in the insoluble form.
Packaging
Package size based on DEA units
Unit Definition
One unit will hydrolyze 1.0 mmole of p-nitrophenyl phosphate per min at pH9.8 at 37 °C.
Physical form
Suspension in 2.0 M (NH4)2SO4, 1 mM MgCl2, 0.1 mM ZnCl2, pH 7
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
动植物源性产品
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Rebika Shrestha et al.
iScience, 25(1), 103608-103608 (2022-02-03)
Understanding the spatiotemporal distribution and dynamics of RAS on the plasma membrane (PM) is the key for elucidating the molecular mechanisms of the RAS signaling pathway. Single particle tracking (SPT) experiments show that in cells, KRAS diffuses in at least
M Kellerer et al.
Diabetologia, 41(7), 833-838 (1998-08-01)
Protein kinase C (PKC) isoforms are potentially important as modulators of the insulin signalling chain and could be involved in the pathogenesis of cellular insulin resistance. We have previously shown that phorbol ester stimulated PKC beta1 and beta2 as well
Li-Na Li et al.
Guang pu xue yu guang pu fen xi = Guang pu, 29(10), 2820-2823 (2009-12-30)
To evaluate the inhibition effect of dansyl-L-phenylalanine on calf intestinal alkaline phosphatase (CIAP), UV-Vis spectrophotometric method was employed. It was found that dansyl-L-phenylalanine can selectively inhibit CIAP. The kinetic inhibition processes of dansyl-L-phenylalanine and L-phenylalanine were comparatively studied. The authors'
J E Coleman
Annual review of biophysics and biomolecular structure, 21, 441-483 (1992-01-01)
Alkaline phosphatase was the first zinc enzyme to be discovered in which three closely spaced metal ions (two Zn ions and one Mg ion) are present at the active center. Zn ions at all three sites also produce a maximally
A Bakker et al.
Journal of virology, 70(8), 5511-5518 (1996-08-01)
The Rex protein is an essential regulator of RNA expression in human T-cell leukemia virus types 1 and 2 (HTLV-1 and HTLV-2) that promotes the accumulation of full-length and partially spliced viral transcripts in the cytoplasm. Rex-mediated regulation correlates with
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
