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NUC201

Sigma-Aldrich

Nuclei Isolation Kit: Nuclei PURE Prep

sufficient for 15 nuclei preparations (~1-10×107 cells or 1g of tissue per preparation)

Synonym(s):

Sucrose centrifugation nuclei isolation

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1 EA
¥6,110.23

¥6,110.23

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1 EA
¥6,110.23

About This Item

UNSPSC Code:
12352207
NACRES:
NA.32

¥6,110.23

Please contact Customer Service for Availability

usage

sufficient for 15 nuclei preparations (~1-10×107 cells or 1g of tissue per preparation)

packaging

pkg of 1 kit

storage condition

dry at room temperature

application(s)

cell analysis

foreign activity

nuclease and protease, free

shipped in

wet ice

storage temp.

2-8°C

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SLB®-5ms Capillary GC Column L × I.D. 20 m × 0.18 mm, df 0.18 μm

28564U

SLB®-5ms Capillary GC Column

SLB®-5ms Capillary GC Column L × I.D. 30 m × 0.25 mm, df 1.00 μm

28476U

SLB®-5ms Capillary GC Column

SLB®-5ms Capillary GC Column L × I.D. 15 m × 0.10 mm, df 0.10 μm

28466U

SLB®-5ms Capillary GC Column

SLB®-5ms Capillary GC Column L × I.D. 30 m × 0.25 mm, df 0.10 μm

28467U

SLB®-5ms Capillary GC Column

df

0.18 μm

df

1.00 μm

df

0.10 μm

df

0.10 μm

parameter

-60-340 °C temperature (isothermal), -60-360 °C temperature (programmed)

parameter

-60-340 °C temperature (isothermal), -60-360 °C temperature (programmed)

parameter

-60-340 °C temperature (isothermal), -60-360 °C temperature (programmed)

parameter

-60-340 °C temperature (isothermal), -60-360 °C temperature (programmed)

material

fused silica

material

fused silica

material

fused silica

material

fused silica

technique(s)

GC/MS: suitable, gas chromatography (GC): suitable (fast GC)

technique(s)

GC/MS: suitable, gas chromatography (GC): suitable

technique(s)

GC/MS: suitable, gas chromatography (GC): suitable (fast GC)

technique(s)

GC/MS: suitable, gas chromatography (GC): suitable

application(s)

agriculture
chemicals and industrial polymers
cleaning products
clinical
cosmetics
environmental
flavors and fragrances
food and beverages
forensics and toxicology
industrial hygiene
life science and biopharma
personal care
petroleum
pharmaceutical (small molecule)

application(s)

agriculture
chemicals and industrial polymers
cleaning products
clinical
cosmetics
environmental
flavors and fragrances
food and beverages
forensics and toxicology
industrial hygiene
life science and biopharma
personal care
petroleum
pharmaceutical (small molecule)

application(s)

agriculture
chemicals and industrial polymers
cleaning products
clinical
cosmetics
environmental
flavors and fragrances
food and beverages
forensics and toxicology
industrial hygiene
life science and biopharma
personal care
petroleum
pharmaceutical (small molecule)

application(s)

agriculture
chemicals and industrial polymers
cleaning products
clinical
cosmetics
environmental
flavors and fragrances
food and beverages
forensics and toxicology
industrial hygiene
life science and biopharma
personal care
petroleum
pharmaceutical (small molecule)

Application

For preparation of pure nuclei and fragile nuclei from cell lines and solid tissues.

Biochem/physiol Actions

The protocol incorporates centrifugation through a dense sucrose cushion to protect nuclei and strip away cytoplasmic contaminants. High yield has been obtained from common cell lines (Jurkat, HFN7.1, COS7, HEK293 and MDCK) and tissues (spleen and liver). These preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones, and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.
The protocol incorporates centrifugation through a dense sucrose cushion to protect nuclei and strip away cytoplasmic contaminants. The sucrose concentration that is suitable for a particular cell type is determined empirically by the user. The sucrose concentrate and sucrose cushion buffer give the user flexibility to modify the density of the sucrose cushion as appropriate. High yield has been obtained from common cell lines (Jurkat, HFN7.1, COS7, HEK293 and MDCK) and tissues (spleen and liver). These preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones, and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.

Kit Components Only

Product No.
Description
  • Nuclei PURE Lysis Buffer 180 mL

related product

Product No.
Description
Pricing

Pictograms

CorrosionEnvironment
GHS05,GHS09

Signal Word

Danger

Hazard Statements

H315,H318,H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    0000349177
    0000349180
    0000344732
    0000337214
    0000331179

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    Analysis of nuclear RNA, Chapter 14.
    Robert E. Farrell, Jr., ed.
    RNA Methodologies: A Laboratory Guide for Isolation and Characterization, 235-263 (1993)
    Nuclei from rat liver: isolation method that combines purity with high yield.
    G Blobel et al.
    Science (New York, N.Y.), 154(3757), 1662-1665 (1966-12-30)
    Michaela Patterson et al.
    Nature genetics, 49(9), 1346-1353 (2017-08-08)
    Adult mammalian cardiomyocyte regeneration after injury is thought to be minimal. Mononuclear diploid cardiomyocytes (MNDCMs), a relatively small subpopulation in the adult heart, may account for the observed degree of regeneration, but this has not been tested. We surveyed 120
    Tyler G Ekins et al.
    eLife, 9 (2020-11-06)
    Type I lissencephaly is a neuronal migration disorder caused by haploinsuffiency of the PAFAH1B1 (mouse: Pafah1b1) gene and is characterized by brain malformation, developmental delays, and epilepsy. Here, we investigate the impact of Pafah1b1 mutation on the cellular migration, morphophysiology
    Ying Xin et al.
    Redox biology, 15, 405-417 (2018-01-22)
    Activation of nuclear factor erythroid 2-related factor 2 (Nrf2) by sulforaphane (SFN) protects from, and deletion of the Nrf2 gene exaggerates, diabetic cardiomyopathy. Angiotensin II (Ang II) plays a critical role in the development of diabetic cardiomyopathy. Therefore, whether SFN
    View All Related Papers

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