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M8662

Sigma-Aldrich

Mineral oil

PCR Reagent

Synonym(s):

Mineral oil for PCR

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1 PKG
CN¥9,436.20

CN¥9,436.20

Estimated to ship onOctober 21, 2025Details

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1 PKG
CN¥9,436.20

About This Item

CAS Number:
8042-47-5
EC Number:
232-455-8
MDL number:
MFCD00131611
UNSPSC Code:
12181504
NACRES:
NA.52
technique(s):
PCR: suitable

CN¥9,436.20

Estimated to ship onOctober 21, 2025Details

form

liquid

packaging

vial of 1.5 mL (Total volume 7.5 mL (5 vials))

technique(s)

PCR: suitable

color

colorless

refractive index

n20/D 1.467 (lit.)

density

0.84 g/mL at 25 °C (lit.)

foreign activity

DNase, RNase, protease, none detected

storage temp.

room temp

InChI key

AEOVEGJBKQQFOP-DDVLFWKVSA-L

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218782187721884
5(6)-Carboxyfluorescein diacetate N-succinimidyl ester BioReagent, suitable for fluorescence, ≥90% (HPLC)

21888

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester

5(6)-Carboxyfluorescein N-hydroxysuccinimide ester BioReagent, suitable for fluorescence, mixture of isomers, ≥80% (HPLC)

21878

5(6)-Carboxyfluorescein N-hydroxysuccinimide ester

5(6)-Carboxyfluorescein suitable for fluorescence, BioReagent, ≥95% (HPLC)

21877

5(6)-Carboxyfluorescein

5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate BioReagent, suitable for fluorescence, ≥85% (HPCE)

21884

5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate

fluorescence

λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase)

fluorescence

λex 492 nm; λem 517 nm in DMF

fluorescence

λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0

fluorescence

λex 470 nm; λem 529 nm in 0.1 M Tris pH 8.0 (esterase)

solubility

DMF: soluble, DMSO: soluble

solubility

DMF: soluble, DMSO: soluble

solubility

-

solubility

DMF: soluble, DMSO: soluble, methanol: soluble

form

solid

form

powder

form

powder

form

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

General description

Mineral oil is used as a reaction mix overlay in polymerase chain reactions (PCR) to prevent reaction solutions from evaporating during the thermal cycling in PCR instruments without heated lids.[1]

Application

Mineral oil has been used:
  • for routine PCR amplifications
  • to improve the heat conductivity between the device and the cycler during reverse transcription-polymerase chain reaction (RT–PCR) amplification[2]
  • to prevent evaporation during single-cell MATQ-sequencing performed on Bravo automated liquid handling platform[3][4]

Features and Benefits

  • Provided in a convenient 5 × 1.5 mL (1 vial) pack size
  • Tested for the absence of DNase, RNase, and protease.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

No data available

Flash Point(C)

No data available

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
  • Specification Sheet

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    Certificates of Analysis (COA)

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    MKCW2105
    MKCN0398
    MKCK1218
    MKCF5776
    MKCC0168

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    Ivan K Dimov et al.
    Nature communications, 5, 3451-3451 (2014-03-29)
    Discriminating cellular heterogeneity is important for understanding cellular physiology. However, it is limited by the technical difficulties of single-cell measurements. Here we develop a two-stage system to determine cellular heterogeneity. In the first stage, we perform multiplex single-cell RNA cytometry
    Hartmut Kaiser et al.
    Plant physiology, 143(2), 1068-1077 (2006-12-13)
    The response of stomata to a reduction of air humidity is composed of a hydropassive opening followed by active closure. Whereas the mechanisms behind the hydropassive opening are largely understood, the location and physiological basis of the sensing mechanisms leading
    Hsin-I Jen et al.
    eLife, 8 (2019-04-30)
    The mammalian cochlea loses its ability to regenerate new hair cells prior to the onset of hearing. In contrast, the adult vestibular system can produce new hair cells in response to damage, or by reprogramming of supporting cells with the
    Decagram scale production of deuterated mineral oil and polydecene as solvents for polymer studies in neutron scattering
    Klenner MA, et al.
    Open Journal of Polymer Chemistry, 11(31), 4986-4994 (2020)
    Neus Bota-Rabassedas et al.
    Cell reports, 35(3), 109009-109009 (2021-04-22)
    Cancer cells function as primary architects of the tumor microenvironment. However, the molecular features of cancer cells that govern stromal cell phenotypes remain unclear. Here, we show that cancer-associated fibroblast (CAF) heterogeneity is driven by lung adenocarcinoma (LUAD) cells at
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    Protocols

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    Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

    Long & Accurate PCR with RedAccuTaq®

    REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.

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    KOD One™ PCR Master Mix overview for ultra-fast PCR with high specificity, fidelity, and yield

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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