21888
5(6)-Carboxyfluorescein diacetate N-succinimidyl ester
BioReagent, suitable for fluorescence, ≥90% (HPLC)
Synonym(s):
CFSE
About This Item
Recommended Products
product line
BioReagent
Quality Level
Assay
≥90% (HPLC)
form
solid
mp
152-154 °C (lit.)
solubility
DMF: soluble
DMSO: soluble
fluorescence
λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase)
suitability
suitable for fluorescence
storage temp.
−20°C
SMILES string
CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(ccc45)C(=O)ON6C(=O)CCC6=O)c1.CC(=O)Oc7ccc8c(Oc9cc(OC(C)=O)ccc9C8%10OC(=O)c%11ccc(cc%10%11)C(=O)ON%12C(=O)CCC%12=O)c7
InChI
1S/2C29H19NO11/c1-14(31)37-17-4-7-21-23(12-17)39-24-13-18(38-15(2)32)5-8-22(24)29(21)20-6-3-16(11-19(20)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34;1-14(31)37-17-4-7-20-23(12-17)39-24-13-18(38-15(2)32)5-8-21(24)29(20)22-11-16(3-6-19(22)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34/h2*3-8,11-13H,9-10H2,1-2H3
InChI key
LZFDYQHOQDFMOA-UHFFFAOYSA-N
General description
Application
- to label cells for flow cytometry
- in lymphocyte proliferation assay
- to determine the intracellular or intra-organellar pH in chloroplasts
- to label Leishmania amazonensis and Leishmania major
Biochem/physiol Actions
Packaging
related product
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
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If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
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At what concentration is Product 21888, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester, soluble in DMSO?
We have found 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester to be soluble in DMSO at a concentration of 1 mg/mL.
What is the ratio between the (5) and (6) isomer of Product 21888, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester?
Our Quality Control group does not routinely report the ratio; however, it is very likely that both isomers are present.
Is Product 21888, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester, light sensitive?
Yes. The powder and solutions (especially solutions) should be kept protected from ambient light whenever possible.
Is Product 21888, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester, fluorescent? Are conjugates of this compound fluorescent?
No. This compound and conjugates of this compound become fluorescent only when acted upon by an enzyme such as esterase. The enzyme removes the acetate groups.
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