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Merck
CN

M8410

Sigma-Aldrich

Mineral oil

light oil, (neat)

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100 μG
CN¥4,472.02

CN¥4,472.02


Available to ship onApril 14, 2025Details


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100 μG
CN¥4,472.02

About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352207
NACRES:
NA.75

CN¥4,472.02


Available to ship onApril 14, 2025Details


Request a Bulk Order

sterility

non-sterile

form

light oil

storage condition

protect from light

refractive index

n20/D 1.467 (lit.)

density

0.84 g/mL at 25 °C (lit.)

format

neat

InChI key

AEOVEGJBKQQFOP-DDVLFWKVSA-L

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This Item
M5904M5310M3516
form

light oil

form

light oil

form

light oil

form

light oil

density

0.84 g/mL at 25 °C (lit.)

density

0.84 g/mL at 25 °C (lit.)

density

0.82-0.88 g/mL at 25 °C

density

0.84 g/mL at 25 °C (lit.)

refractive index

n20/D 1.467 (lit.)

refractive index

n20/D 1.467 (lit.)

refractive index

n20/D 1.467 (lit.)

refractive index

n20/D 1.467 (lit.)

sterility

non-sterile

sterility

-

sterility

sterile-filtered

sterility

-

storage condition

protect from light

storage condition

-

storage condition

protect from light

storage condition

-

Application

Mineral oil has been used as an overlay in microdrop embryo cultures.[1][2][3]

Caution

This product is not sterile. Mineral oil is difficult to sterilize. It can only be sterilized by careful filtration of the warmed oil. It is assumed antibiotics will be added to the cell culture medium. Otherwise, Prod. No. M5310 should be used, as it is sterile filtered.

Analysis Note

This material is tested as an overlay in microdrop embryo cultures. (All embryo tested materials are tested using mouse embryos.) Typically BCF1 x BDF1 hybrids are used. The oil is primarily used to prevent evaporation of the medium, particiularly when performing embryo manipulations.1 The procedure is as follows: 1-cell (BCF1 X BDF1) embryos were cultured in triplicate microdrops of embryo-tested HTF (Human Tubal Fluid) medium supplemented with 0.4% BSA. For a valid assay, at least 70% of 1-cell stage control embryos must develop to the blastocyst stage within 96 hours. For product release, at least 80% of 1-cell stage test embryos must develop to the blastocyst stage within 96 hours with the tested material.

comparable product

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

No data available

Flash Point(C)

No data available

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Minoru Kubo et al.
Nucleic acids research, 47(9), 4539-4553 (2019-03-16)
Next-generation sequencing technologies have made it possible to carry out transcriptome analysis at the single-cell level. Single-cell RNA-sequencing (scRNA-seq) data provide insights into cellular dynamics, including intercellular heterogeneity as well as inter- and intra-cellular fluctuations in gene expression that cannot
Production of a gonadotropin-releasing hormone 2 receptor knockdown (GNRHR2 KD) swine line
Desaulniers AT, et al.
Transgenic research, 26(4), 567-575 (2017)
TIE: A Method to Electroporate Long DNA Templates into Preimplantation Embryos for CRISPR-Cas9 Gene Editing
Bagheri H, et al.
The CRISPR journal, 1(3), 223-229 (2018)
Insulin and branched-chain amino acid depletion during mouse preimplantation embryo culture programmes body weight gain and raised blood pressure during early postnatal life
Velazquez M
Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1864(2), 590-600 (2018)
Mariana Schroeder et al.
Nature communications, 9(1), 1596-1596 (2018-04-25)
Anorexia nervosa (AN) is a devastating eating disorder characterized by self-starvation that mainly affects women. Its etiology is unknown, which impedes successful treatment options leading to a limited chance of full recovery. Here, we show that gestation is a vulnerable

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