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5(6)-Carboxyfluorescein N-hydroxysuccinimide ester

Name: 5(6)-Carboxyfluorescein <I>N</I>-hydroxysuccinimide ester
Brand: SIGMA

BioReagent, suitable for fluorescence, mixture of isomers, ≥80% (HPLC)

Synonym(s):

5(6)-FAM SE, FLUOS

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About This Item

Empirical Formula (Hill Notation):

C₂₅H₁₅NO₉

CAS Number:

117548-22-8

Molecular Weight:

473.39

MDL number:

MFCD04119713

UNSPSC Code:

12352108

PubChem Substance ID:

57647947

NACRES:

NA.32

Quality Level

200

product line

BioReagent

Assay

≥80% (HPLC)

form

powder

solubility

DMF: soluble
DMSO: soluble

fluorescence

λ_ex 492 nm; λ_em 517 nm in DMF

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

OC(=O)c1ccc(cc1C2=C3C=CC(=O)C=C3Oc4cc(O)ccc24)C(=O)ON5C(=O)CCC5=O.OC(=O)c6cccc(C(=O)ON7C(=O)CCC7=O)c6C8=C9C=CC(=O)C=C9Oc%10cc(O)ccc8%10

InChI

1S/2C25H15NO9/c27-13-2-5-16-19(10-13)34-20-11-14(28)3-6-17(20)23(16)18-9-12(1-4-15(18)24(31)32)25(33)35-26-21(29)7-8-22(26)30;27-12-4-6-14-18(10-12)34-19-11-13(28)5-7-15(19)22(14)23-16(24(31)32)2-1-3-17(23)25(33)35-26-20(29)8-9-21(26)30/h1-6,9-11,27H,7-8H2,(H,31,32);1-7,10-11,27H,8-9H2,(H,31,32)

InChI key

YVTPVJDECOUTJW-UHFFFAOYSA-N

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Application

5(6)-Carboxyfluorescein N-hydroxysuccinimide ester (5(6)-FAM SE, FLUOS) may be used to prepare fluorescent derivatives of proteins such as tubulin and for the preparation of a phenytoin tracer for the determination of this and other drugs in serum and plasma by fluorescence polarization immunoassay (FPIA) and laser-induced fluorescence detection.

Fluorescent label for primary and secondary amine groups on proteins and other biomolecules.

Has been used to prepare fluorescent derivatives of tubulin and for the preparation of a phenytoin tracer for the determination of this drug in serum and plasma by fluorescence polarization immunoassay (FPIA)

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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