G4410
Griess reagent (modified)
Synonym(s):
Greiss, nitrite assay
About This Item
General description
Application
- in the nitrite assay of primary spinal cord glial cells stimulated with supernatant of damaged sensory neuron (SDSN)
- for measuring nitrite/nitrate oxidized from nitric oxide in stimulated chondrocytes
- for measuring nitric oxide (NO) degradation in RAW264.7 macrophage cells
- to measure the concentration of nitric oxide(NO) in the conditioned media from cultured macrophages (MΦs)
- to determine the relative levels of NO released by BEAS-2B bronchial cells in the culture supernatants
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1
WGK
WGK 2
Certificates of Analysis (COA)
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
When using Product G4410, Griess' reagent (modified), what product should I use to make a nitrite standard curve?
Sodium nitrite (Product No. S2252 or 237213) can be used to make a standard curve. A typical standard curve would cover the range of 0-100 microMolar nitrite.
Will phenol red interfere with the Griess reaction Product G4410, Griess' reagent (modified)?
While phenol red does not interfere with the Griess reaction, it will interfere with the absorption reading taken at 540nm. It is recommended to use media without phenol red to avoid any interference in the absorption readings.
Is there an enzymatic method to convert nitrate to nitrite?
A coupled assay to convert nitrate to nitrite has been published by Grisham, M.B., et al., Quantitation of nitrate and nitrite in extracellular fluids. Methods Enzymol., 268, 237-246 (1996). For this protocol, we recommend using Product No. N7265 (Nitrate Reductase), Product No.L1378 (L-Lactic Dehydrogenase), Product No.N1630 (NADPH), Product No. P8574 (Sodium pyruvate) and Product No. F6625 (Flavin adenine dinucleotide disodium salt).
What is the difference between Product G4410 Griess reagent (modified), and Product 03553, Griess reagent?
There are many formulations of the Griess Reagent. The formulations for these products are similar; both are proprietary.
How do I find price and availability?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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