11746081001
Roche
Nitrite/Nitrate, Colorimetric Test
sufficient for ~64 assays, pkg of 1 tests (5 components), suitable for water monitoring
Synonym(s):
Nitrate, Nitrite, colormetric test
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About This Item
UNSPSC Code:
12352200
usage
sufficient for ~64 assays
packaging
pkg of 1 tests (5 components)
manufacturer/tradename
Roche
application(s)
water monitoring
storage temp.
2-8°C
Related Categories
General description
Linearity
The method is linear in the range from 0.02 mg nitrite or nitrate/l sample solution to 5.00 mg nitrite or nitrate/l sample solution.
Detection limit: 0.02mg/l for nitrite and nitrate
The method is linear in the range from 0.02 mg nitrite or nitrate/l sample solution to 5.00 mg nitrite or nitrate/l sample solution.
Detection limit: 0.02mg/l for nitrite and nitrate
Specificity
Under the provided conditions, nitrate reductase reacts specifically with nitrate ions.
Application
Semimicro method for the determination of nitrite and/or nitrate to investigate water, waste water, environmental samples, plant material, foodstuffs, drugs, cosmetics, and biological samples. Nitrite/Nitrate, Colorimetric Test was used in a study done to estimate the in vivo effects of myrtle oil (Myrtii oleum) on the antioxidant enzymes such as catalase and superoxide dismutase in alloxan and normoglycaemic diabetic rabbits.
Packaging
1 test combination containing 5 components.
Preparation Note
Working solution: Preparation of the solutions
Storage conditions (working solution):
- Use the contents of bottle #1 undiluted, bring to 15 to 25 °C before use.
- Place one Coenzyme-tablet in a beaker and dissolve in 3 ml of the solution from bottle 1. Remove the Coenzyme-tablet using the tweezers provided. The resulting solution is reaction mixture 2 and is sufficient for 12 nitrate determinations, bring to 15 to 25 °C before use.
- Dissolve the contents of one bottle #3 in 0.7 ml of double-dist. water to give solution 3.
- Use the contents of one bottle #4 undiluted.
- Use the contents of one bottle #5 undiluted.
Storage conditions (working solution):
- Prepare Reaction Mixture 2 immediately before use.
- Solution of Nitrate reductase is stable at 2 to 8 °C for 2 weeks.
- Solution of Color reagent I is stable at 2 to 8 °C for 3 months.
- Solution of Color reagent II is stable at 2 to 8 °C for 3 months.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Kit Components Only
Product No.
Description
- Potassium phosphate buffer, stabilized, pH 7.5
- NADPH, tablets, each containing NADPH and stabilizers
- Nitrate reductase, lyophilized, 8 U
- Color Reagent I, consisting of sulfanilamide and stabilizers
- Color Reagent II, consisting of N-(1-naphthyl)-ethylenediamine dihydrochloride and stabilizers
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
8B - Non-combustible corrosive hazardous materials
WGK
WGK 2
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Regulatory Information
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Emma Gracia-Lor et al.
Chemosphere, 87(5), 453-462 (2012-01-10)
A survey on the presence of pharmaceuticals in urban wastewater of a Spanish Mediterranean area (Castellon province) was carried out. The scope of the study included a wide variety of pharmaceuticals belonging to different therapeutical classes. For this purpose, 112
Aylin Sepici-Dincel et al.
Journal of ethnopharmacology, 110(3), 498-503 (2006-11-15)
In this study we aimed to evaluate the in vivo effects of myrtle oil (myrtii oleum) on the antioxidant enzymes such as superoxide dismutase and catalase, the levels of malondialdehyde in liver tissues as an index of lipid peroxidation and
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