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Merck
CN

11746081001

Roche

Nitrite/Nitrate, Colorimetric Test

sufficient for ~64 assays, pkg of 1 tests (5 components), suitable for water monitoring

Synonym(s):

Nitrate, Nitrite, colormetric test

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.54
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Product Name

Nitrite/Nitrate, Colorimetric Test, sufficient for ~64 assays, pkg of 1 tests (5 components), suitable for water monitoring

usage

sufficient for ~64 assays

packaging

pkg of 1 tests (5 components)

manufacturer/tradename

Roche

application(s)

water monitoring

storage temp.

2-8°C

Application

Semimicro method for the determination of nitrite and/or nitrate to investigate water, waste water, environmental samples, plant material, foodstuffs, drugs, cosmetics, and biological samples. Nitrite/Nitrate, Colorimetric Test was used in a study done to estimate the in vivo effects of myrtle oil (Myrtii oleum) on the antioxidant enzymes such as catalase and superoxide dismutase in alloxan and normoglycaemic diabetic rabbits.

Biochem/physiol Actions

Under the provided conditions, nitrate reductase reacts specifically with nitrate ions.

General description

Linearity
The method is linear in the range from 0.02 mg nitrite or nitrate/l sample solution to 5.00 mg nitrite or nitrate/l sample solution.

Detection limit: 0.02mg/l for nitrite and nitrate

Other Notes

For life science research only. Not for use in diagnostic procedures.

Packaging

1 test combination containing 5 components.

Preparation Note

Working solution: Preparation of the solutions
  • Use the contents of bottle #1 undiluted, bring to 15 to 25 °C before use.
  • Place one Coenzyme-tablet in a beaker and dissolve in 3 ml of the solution from bottle 1. Remove the Coenzyme-tablet using the tweezers provided. The resulting solution is reaction mixture 2 and is sufficient for 12 nitrate determinations, bring to 15 to 25 °C before use.
  • Dissolve the contents of one bottle #3 in 0.7 ml of double-dist. water to give solution 3.
  • Use the contents of one bottle #4 undiluted.
  • Use the contents of one bottle #5 undiluted.

Storage conditions (working solution):
  • Prepare Reaction Mixture 2 immediately before use.
  • Solution of Nitrate reductase is stable at 2 to 8 °C for 2 weeks.
  • Solution of Color reagent I is stable at 2 to 8 °C for 3 months.
  • Solution of Color reagent II is stable at 2 to 8 °C for 3 months.

pictograms

CorrosionHealth hazard

signalword

Danger

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Sens. 1

Storage Class

8B - Non-combustible corrosive hazardous materials

wgk

WGK 2

flash_point_f

does not flash

flash_point_c

does not flash

Regulatory Information

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Emma Gracia-Lor et al.
Chemosphere, 87(5), 453-462 (2012-01-10)
A survey on the presence of pharmaceuticals in urban wastewater of a Spanish Mediterranean area (Castellon province) was carried out. The scope of the study included a wide variety of pharmaceuticals belonging to different therapeutical classes. For this purpose, 112
Aylin Sepici-Dincel et al.
Journal of ethnopharmacology, 110(3), 498-503 (2006-11-15)
In this study we aimed to evaluate the in vivo effects of myrtle oil (myrtii oleum) on the antioxidant enzymes such as superoxide dismutase and catalase, the levels of malondialdehyde in liver tissues as an index of lipid peroxidation and

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