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G2025

Sigma-Aldrich

Glycerol

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for electrophoresis, ≥99% (GC)

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Synonym(s):
1,2,3-Propanetriol, Glycerin
Linear Formula:
HOCH2CH(OH)CH2OH
CAS Number:
56-81-5
Molecular Weight:
92.09
Beilstein:
635685
EC Number:
200-289-5
MDL number:
MFCD00004722
UNSPSC Code:
50161901
PubChem Substance ID:
24895082
NACRES:
NA.21

vapor density

3.1 (vs air)

Quality Level

300

vapor pressure

<1 mmHg ( 20 °C)

product line

BioReagent

Assay

≥99% (GC)

form

viscous liquid

autoignition temp.

698 °F

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable

impurities

≤0.005% Chlorinated compounds
≤0.005% Sulfate (SO4)

refractive index

n20/D 1.474 (lit.)

pH

5.5-8

bp

182 °C/20 mmHg (lit.)

mp

20 °C (lit.)

density

1.25 g/mL (lit.)

suitability

suitable for electrophoresis

SMILES string

OCC(O)CO

InChI

1S/C3H8O3/c4-1-3(6)2-5/h3-6H,1-2H2

InChI key

PEDCQBHIVMGVHV-UHFFFAOYSA-N

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General description

Glycerol is odourless, colorless, viscous in nature, and exists as a sweet tasting liquid. It can be derived naturally as well as from petrochemical feedstock. Glycerol has a wide variety of applications, and is one of the most valuable and versatile chemical substances in nature. It can be used as an emollient, solvent, sweetening agent, in pharmaceutical formulations, cosmetics, foodstuffs and toiletries. It is very stable and can be easily stored under normal temperature; also it is non-irritating and has no adverse impact to the environment.

Application

Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel′s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.

Biochem/physiol Actions

Glycerol has been used as
  • a supplement during cell culture of Mycobacterium tuberculosis and Mycobacterium avium.
  • a fuel during designing enzymatic biofuel cell.
  • a liquid composite matrix with 4-HCCA and 3-amino­quinoline for analysis of neutral and acidic glycans.
  • a matrix for fast atom bombardment MS.
  • may be employed as liquid matrix for the quantification studies by MALDI (Matrix-assisted laser desorption/ionization mass spectrometry) analysis.
Glycerol is hygroscopic in nature and is soluble in water owing to its three hydrophilic alcoholic hydroxyl groups. It can form both inter- and intramolecular hydrogen bonds, making it a very flexible molecule. The physiologic effect of glycerine is due to cell-mediated immunity, increased IgG production and increased histamine release.

WGK

WGK 1

Flash Point(F)

390.2 °F

Flash Point(C)

199 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Is Product No. G2025 a sterile glycerol product?

    Our quality specifications for this product include cell culture testing, but the product is not sterile as it is shipped.

  6. What is the meaning of "Performance" in your specification sheet for Product No. G2025 (Glycerol)?

    The "Performance" test demonstrates that a cell freezing medium prepared using that product maintains the viability of mammalian cells after storing the cells for 5 to 7 days at -196 °C.

  7. What is the concentration of glycerol (e.g., Product No. G2025)?

    Based on a density of 1.261 g/mL for glycerol, and a molecular weight of 92.1 g/mol, the concentration of this liquid is 13.7 M.

  8. Can I autoclave glycerol (e.g., Product No. G2025) before using it (for example) for cell culture?

    Glycerol can be autoclaved for 20 minutes, as is or in a solution, generally at approximately 50%.

  9. In what solvent is glycerol (e.g., Product No. G2025) most soluble?

    Glycerol is miscible with water and alcohol at all concentrations. See: The chemicals encyclopedia published by the Royal Society of Chemistry, 12th ed., Entry# 4493.

  10. What is the viscosity of glycerol?

    The viscosity of glycerol is 648 Centistokes at 20 Deg C (68 Deg F).

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Overview of polyacrylamide gel chemistry and detailed instructions for hand-casting polyacrylamide gels using mPAGE® TurboMix Bis-Tris Gel Casting Kits.

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