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Key Documents

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Safety Information

C7926

Sigma-Aldrich

Collagenase from Clostridium histolyticum

Sigma Blend Type F, ≥2.0 FALGPA units/mg solid

Synonym(s):

Clostridiopeptidase A

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0.5 ML
CN¥2,240.04
1 ML
CN¥4,144.31

CN¥2,240.04

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0.5 ML
CN¥2,240.04
1 ML
CN¥4,144.31

About This Item

CAS Number:
9001-12-1
Enzyme Commission number:
3.4.24.3 (BRENDA, IUBMB)
EC Number:
232-582-9
MDL number:
MFCD00130830
UNSPSC Code:
12352204
NACRES:
NA.54

CN¥2,240.04

Please contact Customer Service for Availability
New, lower price on this item!
Request a Bulk Order

biological source

Clostridium histolyticum

Quality Level

200

form

powder

caseinase activity

≤10 units/mg solid

specific activity

≥2.0 FALGPA units/mg solid

mol wt

68-130 kDa

storage temp.

−20°C

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HNDG4MAG36K

MILLIPLEX® Human Neurodegenerative Disease Magnetic Bead Panel 4 - Neuroscience Multiplex Assay

MILLIPLEX® Human Neuroscience Magnetic Bead Panel 1 - Neuroscience Multiplex Assay allows quantitative multiplex detection of multiple analytes simultaneously

HNS1MAG95K

MILLIPLEX® Human Neuroscience Magnetic Bead Panel 1 - Neuroscience Multiplex Assay

MILLIPLEX® Human Neurological Disorders Magnetic Bead Panel 3 - Neuroscience Multiplex Assay The analytes available for this multiplex kit are: AGT, Contactin-1, Fetuin A, Kallikrein-6, OPN, sSOD1, sSOD2

HND3MAG39K

MILLIPLEX® Human Neurological Disorders Magnetic Bead Panel 3 - Neuroscience Multiplex Assay

MILLIPLEX® Human Neurodegenerative Disease Magnetic Bead Panel 3 - Neuroscience Multiplex Assay The analytes available for this multiplex kit are: BDNF, Cathepsin D, MPO, NCAM, PAI-1 (total), PDGF-AA, PDGF-AB/BB, RANTES, sICAM-1, sVCAM-1.

HNDG3MAG36K

MILLIPLEX® Human Neurodegenerative Disease Magnetic Bead Panel 3 - Neuroscience Multiplex Assay

assay range

accuracy: 84-108%, standard curve range: 21-15,000 pg/mL
(sRAGE), standard curve range: 3-2,500 pg/mL
(Amyloid β40), standard curve range: 27-20,000 pg/mL
(Amyloid β42), standard curve range: 14-10,000 pg/mL
(S100B), standard curve range: 7-5,000 pg/mL
(GDNF)

assay range

accuracy: 107%
(DJ1), accuracy: 108%
(a-Synuclein), accuracy: 123%
(TG2), standard curve range: 12-50,000 pg/mL
(GFAP), standard curve range: 15-60,000 pg/mL
(NSE), standard curve range: 20-80,000 pg/mL
(UCHL1), standard curve range: 244-1,000,000 pg/mL
(alpha-synuclein), standard curve range: 61-250,000 pg/mL
(DJ1), standard curve range: 61-250,000 pg/mL
(TG2)

assay range

accuracy: 69-92%
(Cerebrospinal fluid (CSF)), accuracy: 71-107%
(Serum), standard curve range: 0.03-25 ng/mL
(SOD2), standard curve range: 0.04-30 ng/mL
(Kallikrein-6), standard curve range: 0.04-30 ng/mL
(SOD1), standard curve range: 0.08-60 ng/mL
(Contactin-1), standard curve range: 0.14-100 ng/mL
(Osteopontin (OPN)), standard curve range: 1.37-1,000 ng/mL
(AGT), standard curve range: 4.12-3,000 ng/mL
(Fetuin A)

assay range

accuracy: 108%
(RANTES), accuracy: 108%
(sICAM-1), accuracy: 111%
(Cathepsin D), accuracy: 123%
(PDGF-AA), accuracy: 137%
(PDGF-AB/BB), standard curve range: 2-10,000 pg/mL
(RANTES), standard curve range: 2.4-10,000 pg/mL
(BDNF), standard curve range: 2.4-10,000 pg/mL
(PAI-1 (total)), standard curve range: 2.4-10,000 pg/mL
(PDGF-AA), standard curve range: 24-100,000 pg/mL
(Cathepsin D), standard curve range: 24-100,000 pg/mL
(MPO), standard curve range: 24-100,000 pg/mL
(NCAM), standard curve range: 24-100,000 pg/mL
(PDGF-AB/BB), standard curve range: 24-100,000 pg/mL
(sICAM-1), standard curve range: 61-250,000 pg/mL
(sVCAM-1)

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

Application

Collagenase from Clostridium histolyticum has been used in determining the degradation rate of collagen biomaterials.[1] It may also be used to determine the enzymatic degradation and enzymatic resistance by the liberated L-leucine measurements.[2]
Collagenase from Clostridium histolyticum, or Clostridiopeptidase A, has been used in a study to assess contact dermatitis with clostridiopeptidase A contained in Noruxol ointment. Clostridiopeptidase A has also been used in a study to investigate the early and successful enzymatıc debridement via collagenase application to pinna in a preterm neonate.
The enzyme has been used in the isolation of mast cells from adult mice. This study generated a large number of connective tissue-type mast cells by the culture of murine fetal skin cells.[3] The product has also been used in the in vitro biodegradation study of Bombyx mori silk fibroin fibers and films, by exposing them to the enzyme for varied time periods.[4]

Biochem/physiol Actions

Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.
Effective release of cells from tissue requires the action of collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca2+) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA)4; β-mercaptoethanol; glutathione, reduced; thioglycolic acid, sodium; and 2,2′-dipyridyl; 8-hydroxyquinoline are known to inhibit the enzyme activity.

Unit Definition

One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H334,H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000409930
    0000348117
    0000317521
    0000317521
    0000409930

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    Biodegradation of Bombyx mori silk fibroin fibers and films.
    Arai T, et al.
    Journal of Applied Polymer Science, 91(4), 2383-2390 (2004)
    Nobuo Yamada et al.
    The Journal of investigative dermatology, 121(6), 1425-1432 (2003-12-17)
    We describe a novel culture system for generating large numbers of murine skin-associated mast cells and distinguish their characteristics from bone marrow-derived cultured mast cells. Culture of day 16 fetal skin single cell suspensions in the presence of interleukin-3 and
    Properties of collagen and hyaluronic acid composite materials and their modification by chemical crosslinking
    Rehakova M, et al.
    Journal of Biomedical Materials Research, 30(3), 369-372 (1996)
    K Vizárová et al.
    Biomaterials, 16(16), 1217-1221 (1995-11-01)
    Two kinds of layered atelocollagen materials cross-linked with hexamethylene diisocyanate (HMDIC), starch dialdehyde and glyoxal were enzymatically treated by bacterial collagenase. Evaluating collagenase digestion assay for these material showed progressive differences, particularly in the group of samples cross-linked with HMDIC.
    Brittney R Garner et al.
    The Journal of pharmacology and experimental therapeutics, 376(1), 40-50 (2020-10-27)
    Pharmacological openers of ATP-sensitive potassium (KATP) channels are effective antihypertensive agents, but off-target effects, including severe peripheral edema, limit their clinical usefulness. It is presumed that the arterial dilation induced by KATP channel openers (KCOs) increases capillary pressure to promote
    View All Related Papers

    Protocols

    Enzymatic Assay of Collagenase (EC 3.4.24.3) using FALGPA (N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala)

    To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.

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