C0739
Anti-phospho-Cortactin (pTyr421) antibody produced in rabbit
affinity isolated antibody, buffered aqueous glycerol solution
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About This Item
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous glycerol solution
mol wt
antigen 80-85 kDa
species reactivity
mouse
technique(s)
immunocytochemistry: suitable
western blot: 1:1,000
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pTyr421)
Gene Information
mouse ... Cttn(13043)
General description
Cortactin is encoded by the CTTN gene on chromosome 11 in humans. It is a cytoskeleton protein that facilitates assembly of cortical actin. It is expressed in most adherent cells and is a prominent substrate of protein-tyrosine kinase Src.
Immunogen
synthetic phosphopeptide derived from the region of mouse cortactin that is phosphorylated on tyrosine 421.
Application
Anti-phospho-Cortactin (pTyr421) antibody produced in rabbit is suitable for immunocytochemistry and western blotting at a working dilution of 1:1,000
Biochem/physiol Actions
Cortactin is activated by phosphorylation of tyrosine kinases or serine/threonine kinases. It plays an important role in promoting lamellipodia formation, invadopodia formation, cell migration, and endocytosis. Cortactin regulates the interactions between components of adherens-type junctions and helps in organizing the cytoskeleton, cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. It acts as a regulator in invasion and migration of tumor cells. The overexpression of cortactin causes osteosarcoma carcinogenesis and along with FAK increases the risk of oral premalignant lesions.
Physical form
Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL bovine serum albumin (IgG and protease free) and 0.05% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Maxwell D Weidmann et al.
Scientific reports, 6, 36142-36142 (2016-11-09)
Invadopodia, actin-based protrusions of invasive carcinoma cells that focally activate extracellular matrix-degrading proteases, are essential for the migration and intravasation of tumor cells during dissemination from the primary tumor. We have previously shown that cortactin phosphorylation at tyrosine residues, in
Cortactin is a sensitive biomarker relative to the poor prognosis of human hepatocellular carcinoma.
Gang Zhao et al.
World journal of surgical oncology, 11, 74-74 (2013-03-23)
Cortactin is an important regulator involved in invasion and migration of hepatocellular carcinoma (HCC). The aim of this study was to elucidate the forecasting role of cortactin in resectable HCCs. We compared the invasiveness and motility among liver epithelial cell
Cortactin (CTTN) overexpression in osteosarcoma correlates with advanced stage and reduced survival.
Cecilia Folio et al.
Cancer biomarkers : section A of Disease markers, 10(1), 35-41 (2012-02-03)
The cortactin (CTTN) gene has been found, by transcriptomic profiling, to be overexpressed in pediatric osteosarcoma. The location of CTTN at 11q13 and the role of cortactin in cytoskeleton restructuring make CTTN of interest as a potential biomarker for osteosarcoma.
Roger J Daly
The Biochemical journal, 382(Pt 1), 13-25 (2004-06-10)
Cortactin was first identified over a decade ago, and its initial characterization as both an F-actin binding protein and v-Src substrate suggested that it was likely to be a key regulator of actin rearrangements in response to tyrosine kinase signalling.
Kellye C Kirkbride et al.
Cytoskeleton (Hoboken, N.J.), 69(9), 625-643 (2012-09-20)
Cortactin is a branched actin regulator and tumor-overexpressed protein that promotes vesicular trafficking at a variety of cellular sites, including endosomes and the trans-Golgi network. To better understand its role in secretory trafficking, we investigated its function in Golgi homeostasis.
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