B6755
BIS-TRIS propane
≥99.0% (titration)
Synonym(s):
1,3-Bis[tris(hydroxymethyl)methylamino]propane, 2,2′-(Propane-1,3-diylbis(azanediyl))bis(2-(hydroxymethyl)propane-1,3-diol)
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About This Item
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Quality Level
Assay
≥99.0% (titration)
form
powder
storage condition
dry at room temperature
technique(s)
HPLC: suitable
color
white
useful pH range
6.3-9.5
pKa (25 °C)
(1) 6.8, (2) 9.0
mp
164-165 °C (lit.)
solubility
water: 30 % (w/w), clear, colorless
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1 of 4
This Item | F8636 | F9378 | F2637 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| product line BioReagent | product line BioXtra | product line - | product line BioXtra |
| type Type 400-DL | type - | type Type 400-DL | type - |
organelle preparation, centrifugation gradients | organelle preparation, centrifugation gradients | - | - |
General description
Furthermore, Bis-Tris Propane is a water-soluble buffer substance that plays a crucial role in enhancing the stability and activity of restriction enzymes. Specifically, its buffering capacity is advantageous for experiments that require pH levels as low as 6-7. When compared to Tris buffer, which has a poor buffering capacity below pH 7.5 and exhibits significant pKa fluctuations with temperature changes, Bis-Tris Propane proves to be a superior choice. Additionally, it finds application in conjunction with hydrochloric acid buffer to stabilize farnesyl diphosphate, particularly when isolated from Saccharomyces cerevisiae strains. Notably, it can also serve as a ligand, forming dinuclear hydroxo complexes with Lanthanides(III).
Application
- as a buffer to investigate the influence of anions with varying nucleophilic properties on the autoxidation of oxymyoglobin (MbO2).[1]
- for purification of glucose-binding protein from membranes of Sulfolobus solfataricus.[2]
- as a buffer during GeO2 mineralisation.[3]
- as a buffer component in a study pertaining to environmental research[4]
- as a buffer component to maintain optimal pH conditions during the purification and preparation of engineered enzymes[5]
Features and Benefits
- Suitable for Biological and Biochemical Research
- Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
- Effective Buffering from pH 6.3-9.5 (25 °C) with a pKa of 6.8 and 9.0 (25 °C)
- Highly soluble in water
Other Notes
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
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