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Key Documents

Safety Information

A3641

Sigma-Aldrich

Apoferritin from equine spleen

0.2 μm filtered

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100 μL
CN¥5,551.31

CN¥5,551.31

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100 μL
CN¥5,551.31

About This Item

CAS Number:
9013-31-4
MDL number:
MFCD00081365
UNSPSC Code:
12352202
NACRES:
NA.32

CN¥5,551.31

Please contact Customer Service for Availability
Request a Bulk Order

Quality Level

200

sterility

0.2 μm filtered

form

liquid

mol wt

major subunit ML 19,889
minor subunit MH 22,200
native ~481.2 kDa (24 subunits, approx. 20 kDa each)

color

clear to slightly hazy, solution

storage temp.

2-8°C

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PHR1533C8503C8667
胆固醇 powder, BioReagent, suitable for cell culture, ≥99%

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胆固醇 Pharmaceutical Secondary Standard; Certified Reference Material

PHR1533

胆固醇

胆固醇 from sheep wool, ≥92.5% (GC), powder

C8503

胆固醇

胆固醇 Sigma Grade, ≥99%

C8667

胆固醇

technique(s)

cell culture | mammalian: suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

-

technique(s)

-

assay

≥99%

assay

-

assay

≥92.5% (GC)

assay

≥99%

biological source

ovine wool

biological source

-

biological source

sheep wool

biological source

sheep wool

solubility

H2O: 0.002 mg/mL, ethanol: soluble, Tween: soluble, serum: soluble

solubility

water: soluble 0.00003 g/L at 20 °C

solubility

water: soluble 0.00003 g/L at 20 °C

solubility

water: soluble 0.00003 g/L at 20 °C

form

powder

form

powder

form

powder

form

powder

storage temp.

−20°C

storage temp.

-10 to -25°C

storage temp.

room temp

storage temp.

−20°C

Application

Apoferritin from equine spleen has been used:
  • to determine its partition coefficients in phase systems[1]
  • in NaCl for iron loading of cultured cells[2]
  • in in situ liquid scanning transmission electron microscope for imaging the interface of biology and nanotechnology[3]

Biochem/physiol Actions

This protein shell of ferritin lacking iron is widely used for the calibration of gel filtration columns and sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis.[4] It is very abundant in beta-cells of the pancreas where it acts as an anti-oxidant.[5] When added to cultured endothelial cells apoferritin is taken up in a dose-responsive manner and appears to protect the cells from oxidant-mediated cytolysis. [6]

Physical form

Solution in 0.135 M sodium chloride.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

动植物源性产品
  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000408416
    0000368426
    0000344224
    0000340133
    0000316876

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    Find documentation for the products that you have recently purchased in the Document Library.

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    Visualizing macromolecular complexes with in situ liquid scanning transmission electron microscopy
    Evans JE, et al.
    Micron (Oxford, England : 1993), 43(11), 1085-1090 (2012)
    Ferritin-iron increases killing of Chinese hamster ovary cells by X-irradiation
    Nelson JM and Stevens RG
    Cell proliferation, 25(6), 579-585 (1992)
    Jorge H Melillo et al.
    Scientific reports, 12(1), 16512-16512 (2022-10-04)
    Some of the best nucleating agents in nature are ice-nucleating proteins, which boost ice growth better than any other material. They can induce immersion freezing of supercooled water only a few degrees below 0 °C. An open question is whether this
    Hamish G Brown et al.
    Communications biology, 5(1), 817-817 (2022-08-15)
    Ice thickness is arguably one of the most important factors limiting the resolution of protein structures determined by cryo-electron microscopy (cryo-EM). The amorphous atomic structure of the ice that stabilizes and protects biological samples in cryo-EM grids also imprints some
    G Balla et al.
    The Journal of biological chemistry, 267(25), 18148-18153 (1992-09-05)
    Phagocyte-mediated oxidant damage to vascular endothelium is likely involved in various vasculopathies including atherosclerosis and pulmonary leak syndromes such as adult respiratory distress syndrome. We have shown that heme, a hydrophobic iron chelate, is rapidly incorporated into endothelial cells where
    View All Related Papers

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