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Merck
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Key Documents

A0410

Sigma-Aldrich

Aminoallyl-dUTP sodium salt

≥85%, lyophilized powder

Synonym(s):

5-[3-Aminoallyl]-2′-deoxyuridine 5′-triphosphate, AA-dUTP

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About This Item

Empirical Formula (Hill Notation):
C12H20N3O14P3 · xNa+
CAS Number:
936327-10-5
Molecular Weight:
523.22 (free acid basis)
MDL number:
MFCD06200396
UNSPSC Code:
41106305
NACRES:
NA.51
To inquire about this product A0410, please contact your local Merck office or dealer. Contact Technical Service

Quality Level

200

Assay

≥85%

form

lyophilized powder

shipped in

dry ice

storage temp.

−70°C

SMILES string

[P](=O)(O[P](=O)(OCC1OC(CC1O)N2C=C(C(=O)NC2=O)\C=C\CN)O)(O[P](=O)(O)O)O.N

InChI key

YWKTUHBZWBRYHX-TYYBGVCCSA-N

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biological source

rabbit

biological source

mouse

biological source

rabbit

biological source

rabbit

antibody form

affinity purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity purified immunoglobulin

antibody form

affinity purified immunoglobulin

UniProt accession no.

Q13936

UniProt accession no.

Q13936

UniProt accession no.

Q01668

UniProt accession no.

O00555

clone

polyclonal

clone

8F9.1, monoclonal

clone

polyclonal

clone

polyclonal

technique(s)

immunohistochemistry: suitable, western blot: suitable, immunoprecipitation (IP): suitable

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

Application

Aminoallyl-dUTP may be used for indirect stable labeling of first-strand cDNA synthesis via polymerase chain reaction (PCR). Aminoallyl-dUTP functions as a reactive site for subsequent labeling by reactive fluorescent probes. Aminoallyl-dUTP may also be useful in the construction of reactive DNA scaffolds.
Aminoallyl-dUTP sodium salt has been used:
  • to add to the deoxynucleoside triphosphate mix for complementary DNA (cDNA) synthesis[1]
  • for the generation of chromatin immunoprecipitation (ChIP) probes[2]
  • in the reverse transcription of RNA for the synthesis of fluorescent labelled probes[3][4]

Biochem/physiol Actions

Aminoallyl-dUTP sodium salt/5-(3-aminoallyl)-2′-deoxyuridine 5′-triphosphate (aa-dUTP) have been used mainly in two-step labeling approach for the synthesis of nucleic acid hybridisation probe.[5]

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000379569
    0000344576
    0000338589
    0000333957
    0000326415

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    Lorenzo Berti et al.
    Nanotechnology, 20(23), 235101-235101 (2009-05-19)
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    Miguel A Matilla et al.
    Genome biology, 8(9), R179-R179 (2007-09-06)
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    Light is a pervasive environmental factor that regulates development, stress resistance, and even virulence in numerous fungal species. Though much research has focused on signaling pathways in Aspergillus fumigatus, an understanding of how this pathogen responds to light is lacking.
    Effect of the Colorless non-ripening mutation on cell wall biochemistry and gene expression during tomato fruit development and ripening
    Eriksson E M, et al.
    Plant Physiology, 136(4), 4184-4197 (2004)
    Chromatin immunoprecipitation (ChIP) on chip experiments uncover a widespread distribution of NF-Y binding CCAAT sites outside of core promoters
    Testa A, et al.
    The Journal of Biological Chemistry, 280(14), 13606-13615 (2005)
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