S9430
SYBR® Green I nucleic acid gel stain
10,000 × in DMSO
Synonym(s):
DNA stain, SYBR® green gel dye, safer gel stain
About This Item
form
solution
Quality Level
usage
mL sufficient for 100 mini-gels
greener alternative product characteristics
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sustainability
Greener Alternative Product
concentration
10,000 × in DMSO
technique(s)
PCR: suitable
greener alternative category
storage temp.
−20°C
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General description
Application
- the quantification of dsDNA
- to stain DNA in polymerase chain reaction (PCR)
- for comet assay technique
- to assess spermatozoon membrane integrity
- for visual inspection of DNA amplified by loop-mediated isothermal amplification (LAMP)
- as a fluorescent dye in flow cytometry
- real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for staining reverse-transcribed cDNA
Features and Benefits
- An ultrasensitive stain for post-electrophoresis staining of dsDNA in agarose or polyacrylamide gels
- It can also detect ssDNA and RNA in denaturing agarose/formaldehyde and polyacrylamide/urea gels without any pre-washing steps
- It is less mutagenic than ethidium bromide in Ames tests
- It provides 50-100 times greater detection sensitivity than ethidium bromide for oligonucleotides
- Useful for many applications with a limited amount of DNA
- The binding of SYBR® Green I to DNA does not inhibit the activity of many common restriction endonucleases, including Hind III and EcoR I
- Removal of this stain in-gel digestion and ligation techniques is not needed
- SYBR Green I is a greener alternative product to ethidium bromide for staining
Storage and Stability
Legal Information
related product
WGK
WGK 3
Flash Point(F)
closed cup
Flash Point(C)
closed cup
Personal Protective Equipment
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Will Product S9430, SYBR® Green I nucleic acid gel stain, stain DNA that contains deaza-G modified nucleotides in place of guanisine?
Product S9430, SYBR® Green I nucleic acid gel stain, will poorly stain DNA containing deaza-G modified nucleotides in place of guanisine. A way around this issue would be to use a mixture of deaza-G modified nucleotides with guanisine. This will allow for better staining.
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