S9430
SYBR® Green I nucleic acid gel stain
10,000 × in DMSO
Synonym(s):
DNA stain, SYBR® green gel dye, safer gel stain
Select a Size
CN¥397.95
Estimated to ship onApril 22, 2025Details
Select a Size
About This Item
CN¥397.95
Estimated to ship onApril 22, 2025Details
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form
solution
Quality Level
usage
1.0 mL sufficient for 100 mini-gels
greener alternative product characteristics
Designing Safer Chemicals
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sustainability
Greener Alternative Product
concentration
10,000 × in DMSO
technique(s)
PCR: suitable
greener alternative category
storage temp.
−20°C
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This Item | EZHPI | EZHCP | EZHGIP |
|---|---|---|---|
| assay range accuracy: 76-103%, standard curve range: 2-200 μU/mL, linearity: 96-112% | assay range accuracy: 88-103%, linearity: 84-120%, sensitivity: 0.5 pM, standard curve range: 2-200 pM | assay range accuracy: 91-111%, linearity: 86-108%, standard curve range: 0.2-20 ng/mL | assay range accuracy: 86.7%, linearity: 99.9%, sensitivity: 8.2 pg/mL, standard curve range: 8.2-2000 pg/mL |
| input sample type plasma (K2 EDTA) | input sample type serum | input sample type plasma (K2 EDTA) | input sample type cell culture supernatant |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| technique(s) ELISA: suitable | technique(s) ELISA: suitable | technique(s) ELISA: suitable | technique(s) ELISA: suitable |
| detection method colorimetric (450nm) | detection method colorimetric (450nm) | detection method colorimetric (450nm) | detection method colorimetric (450nm/590nm) |
General description
Application
- the quantification of dsDNA[3]
- to stain DNA in polymerase chain reaction (PCR)[4]
- for comet assay technique[5]
- to assess spermatozoon membrane integrity[6]
- for visual inspection of DNA amplified by loop-mediated isothermal amplification (LAMP)[7]
- as a fluorescent dye in flow cytometry[8]
- real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for staining reverse-transcribed cDNA[8]
Features and Benefits
- An ultrasensitive stain for post-electrophoresis staining of dsDNA in agarose or polyacrylamide gels
- It can also detect ssDNA and RNA in denaturing agarose/formaldehyde and polyacrylamide/urea gels without any pre-washing steps
- It is less mutagenic than ethidium bromide in Ames tests
- It provides 50-100 times greater detection sensitivity than ethidium bromide for oligonucleotides
- Useful for many applications with a limited amount of DNA
- The binding of SYBR® Green I to DNA does not inhibit the activity of many common restriction endonucleases, including Hind III and EcoR I
- Removal of this stain in-gel digestion and ligation techniques is not needed
- SYBR Green I is a greener alternative product to ethidium bromide for staining
Storage and Stability
Legal Information
related product
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
201.2 °F - closed cup
Flash Point(C)
94 °C - closed cup
Personal Protective Equipment
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If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
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The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Will Product S9430, SYBR® Green I nucleic acid gel stain, stain DNA that contains deaza-G modified nucleotides in place of guanisine?
Product S9430, SYBR® Green I nucleic acid gel stain, will poorly stain DNA containing deaza-G modified nucleotides in place of guanisine. A way around this issue would be to use a mixture of deaza-G modified nucleotides with guanisine. This will allow for better staining.
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