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D0426

Sigma-Aldrich

DAB with Metal Enhancer

SIGMAFAST™, peroxidase substrate, tablet

Synonym(s):

3,3′-Diaminobenzidine Peroxidase Substrate, DAB Peroxidase Substrate

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.83

product name

SIGMAFAST™ DAB with Metal Enhancer, tablet

form

tablet

Quality Level

200

solubility

water: 1 tablet/5 mL

storage temp.

−20°C

Related Categories

General description

SIGMA FAST™ DAB with Metal Enhancer Tablets require no additional buffers or steps to prepare an active substrate solution for detection of peroxidase activity. Commom use is in immunohistology as a precipitating substrate.

Application

SIGMAFAST™ DAB with Metal Enhancer has been used in the visualization of lung tissue sections using immunohistochemistry, dorsal root ganglion (DRG) neurons and immunohistochemical staining for collagen in anterior cruciate ligament (ACL) cells.
The DAB reaction is enhanced by the addition of CoCl2 and produces a more intense stain which is resistant to alcohol.

Reconstitution

Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing DAB with a metal enhancer.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Hazard Classifications

Aquatic Chronic 2 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the metal enhancer in Product D0426, SIGMAFAST™ DAB with Metal Enhancer, and what is the purpose of it?

    The metal enhancer is cobalt chloride (CoCl2). Cobalt chloride produces a more intense stain which enhances the sensitivity of the assay and allows for visualization of the staining when a standard DAB chromogen would fail to demonstrate the staining.

  4. What is a tablet set for Product D0426, SIGMAFAST™ DAB with Metal Enhancer?

    This product contains two substrate tablets per tablet set; there is one DAB/cobalt tablet and one urea/hydrogen peroxide tablet. Both tablets must be used in the assay.

  5. How can I block endogenous peroxidase activity when using Product D0426, SIGMAFAST™ DAB with Metal Enhancer, in immunohistochemistry?

    Endogenous peroxidase activity may be blocked by flooding the slide with a solution of 4 parts methanol and 1 part 3% hydrogen peroxide.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

M Breijo et al.
Journal of economic entomology, 110(3), 1390-1393 (2017-04-08)
The horn fly, Haematobia irritans (L.), is a blood-sucking livestock ectoparasite responsible for substantial livestock losses. In the present work, the potential use of recombinant hematobin (HTB), a horn fly salivary protein, as an antigen for cattle vaccination was investigated.
Saba Shahin et al.
Toxicological sciences : an official journal of the Society of Toxicology, 161(2), 349-374 (2017-10-27)
Microwave (MW) radiation induced oxidative stress reduces dendritic arborization, spine density and number of hippocampal pyramidal neurons and hence, impair learning and spatial memory through p53-dependent/independent apoptosis of hippocampal neuronal and nonneuronal cells. However, the mechanisms responsible for MW radiation
Somanshu Banerjee et al.
Journal of photochemistry and photobiology. B, Biology, 175, 254-268 (2017-09-20)
Birds time their daily and seasonal activities in synchronization with circadian and annual periodicities in the environment, which is mainly provided by changes in photoperiod/day length conditions. Photoperiod appears to act at the level of eye, pineal and encephalic/deep brain
Developing rat lung has a sided pacemaker region for morphogenesis-related airway peristalsis
Jesudason EC, et al.
American Journal of Respiratory Cell and Molecular Biology, 32(2), 118-127 (2005)
Cdk5 inhibitor roscovitine alleviates neuropathic pain in the dorsal root ganglia by downregulating N-methyl-D-aspartate receptor subunit 2A
Yang L, et al.
Neurological Sciences, 35(9), 1365-1371 (2014)
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NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Protocols

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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