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D4293

Sigma-Aldrich

3,3′-Diaminobenzidine

SIGMAFAST™, chromogenic, tablet

Synonym(s):

DAB tablets

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About This Item

CAS Number:
91-95-2
MDL number:
MFCD00007725
UNSPSC Code:
12352204
PubChem Substance ID:
24893856
NACRES:
NA.83

Product Name

SIGMAFAST™ 3,3′-Diaminobenzidine tablets, tablet, To prepare 5 mL

Quality Level

200

form

tablet

storage temp.

−20°C

SMILES string

Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2

InChI key

HSTOKWSFWGCZMH-UHFFFAOYSA-N

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This Item
756385438790373826
Zirconium yttrium alloy sputtering target, diam. × thickness 2.00 in. × 0.25 in., 99.9% trace metals basis (excluding <=1% Hf)

774057

Zirconium yttrium alloy

锆 powder, in water, -325 mesh, 99.5% trace metals basis (excluding Hf)

756385

锆

乙酰丙酮钇(III) 水合物 99.95% trace metals basis

438790

乙酰丙酮钇(III) 水合物

三(2,2,6,6-四甲基-3,5-庚二酮酸)钇(III) 99.9% trace metals basis

373826

三(2,2,6,6-四甲基-3,5-庚二酮酸)钇(III)

form

solid

form

powder

form

powder or crystals

form

solid

assay

99.9% trace metals basis (excluding <=1% Hf)

assay

99.5% trace metals basis (excluding Hf)

assay

99.95% trace metals basis

assay

99.9% trace metals basis

diam. × thickness

2.00 in. × 0.25 in.

diam. × thickness

-

diam. × thickness

-

diam. × thickness

-

Application

Developed for use in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. DAB is the immunohistology substrate of choice as it produces an intense brown-black stain which is resistant to alcohol. Slides stained with DAB may be dehydrated, mounted in resinous media, and stored for future reference. SIGMAFAST™ DAB Tablets require no additional buffers or steps to prepare an active substrate solution. Tablets are individually packaged in foil packets
SIGMAFAST™ 3,3′-Diaminobenzidine tablets has been used as a chromogen for visualization in immunohistochemistry of gonad tissue,[1] thymus,[2] dental chronic periapical lesions[3] and gall bladder sections.[4]

Reconstitution

Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing DAB and urea hydrogen peroxide.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Hazard Statements

H315,H318,H335,H341,H350

Hazard Classifications

Carc. 1B - Eye Dam. 1 - Muta. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    SLCM3825
    SLCG5357
    SLCD1662
    SLBX4976
    SLBR2973V

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    Increased Bax/Bcl-2 ratio up-regulates caspase-3 and increases apoptosis in the thymus of patients with myasthenia gravis
    Salakou S, et al.
    In Vivo, 21(1), 123-132 (2007)
    Interstitial cells of Cajal are present in human extrahepatic bile ducts
    Ahmadi O, et al.
    Journal of Gastroenterology and Hepatology, 25(2), 277-285 (2010)
    Receptor activator NFkappaB-ligand and osteoprotegerin protein expression in human periapical cysts and granulomas
    Menezes R, et al.
    Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics, 102(3), 404-409 (2006)
    Expression of pluripotent stem cell markers in the human fetal testis
    Kerr CL, et al.
    Stem Cells, 26(2), 412-421 (2008)
    Laura Gribouval et al.
    Scientific reports, 8(1), 6942-6942 (2018-05-04)
    Nanos are RNA-binding proteins playing crucial roles in germ cell development and maintenance. Based on phylogenetic and synteny analyses, this study reveals that nanos1 gene has undergone multiple duplications and gene copies losses in Vertebrates. Chondrichthyan species display two nanos1
    View All Related Papers

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    Colorimetric Phosphatase/Peroxidase Substrate Systems

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