D5637
3,3′-Diaminobenzidine tetrahydrochloride hydrate
≥96%
Synonym(s):
(1,1′-Biphenyl)-3,3′,4,4′-tetramine tetrahydrochloride dihydrate
About This Item
Quality Level
Assay
≥96%
form
powder, crystals or chunks
technique(s)
titration: 96-104% (with NaOH)
color
white to light brown, and Faint Red and Faint Grey to Light Grey
mp
280 °C
solubility
H2O: 50 mg/mL
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
−20°C
SMILES string
O.Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2
InChI
1S/C12H14N4.4ClH.H2O/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;;/h1-6H,13-16H2;4*1H;1H2
InChI key
DXWSCXIZIHILNP-UHFFFAOYSA-N
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Related Categories
General description
Application
Biochem/physiol Actions
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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What is Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB), soluble in?
DAB HCl is soluble in water at 50 mg/ml yielding a clear to slightly hazy solution. For consistency, it is important to allow DAB to come to room temperature before making solutions. Water quality is extremely important, so only high quality deionized or distilled water should be used to make DAB solutions.
What is the solution stability of Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB)?
In our experience, solutions of DAB should be made immediately prior to use for best results. If and only if necessary, solutions can be kept in the dark and refrigerated for overnight storage. Based on our Immunology Department’s experience, storing the solution overnight did not give good reproducibility. However, the literature indicates that it is possible to store solutions of DAB at -20°C. DAB dissolved in Tris buffered saline (pH 7.6) at 0.6 mg/ml was reported to be stable for several weeks at 4°C.
Can the solutions of Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB), be filtered?
DAB solution may be filtered through a 0.2 micron filter immediately prior to use.
Do you have a staining protocol for Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB)?
Dissolve 10 mg in 15 mL of Tris buffered saline, pH 7.6 (Tris-buffered saline tablet, Sigma Product No. T5030 may be used). Add 12 uL of fresh 30% hydrogen peroxide (Sigma Product No. H1009) prior to use. Solution may be filtered through a 0.2 micron filter immediately prior to use if necessary. DAB and hydrogen peroxide concentrations may be adjusted to suit individual applications. The stain can be enhanced with Ni. Make up 0.3% (w/v) NiCl2 in water. Add 1 mL of the NiCl2 stock to 9 mL DAB solution.
What is the shelf life of Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB)?
This product has a shelf life of 3 years.
How is Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB), shipped and stored?
The product is shipped at ambient temperature. However, it is stored at -20 degrees C for long term storage.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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