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28562

Sigma-Aldrich

Atto 488 maleimide

BioReagent, suitable for fluorescence, ≥90% (HPLC)

Synonym(s):

Atto 488

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25 MG
CN¥4,549.28
100 MG
CN¥11,969.92

CN¥4,549.28

Estimated to ship onApril 07, 2025Details

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25 MG
CN¥4,549.28
100 MG
CN¥11,969.92

About This Item

MDL number:
MFCD07784936
UNSPSC Code:
12352111
NACRES:
NA.32

CN¥4,549.28

Estimated to ship onApril 07, 2025Details

Request a Bulk Order

product line

BioReagent

Assay

≥90% (HPLC)
≥90% (degree of coupling )

manufacturer/tradename

ATTO-TEC GmbH

λ

in methanol: water (1:1) (with 0.1% perchloric acid)

UV absorption

λ: 501-506 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

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5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate BioReagent, suitable for fluorescence, ≥85% (HPCE)

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fluorescence

λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase)

fluorescence

λex 492 nm; λem 517 nm in DMF

fluorescence

λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0

fluorescence

λex 470 nm; λem 529 nm in 0.1 M Tris pH 8.0 (esterase)

solubility

DMF: soluble, DMSO: soluble

solubility

DMF: soluble, DMSO: soluble

solubility

-

solubility

DMF: soluble, DMSO: soluble, methanol: soluble

form

solid

form

powder

form

powder

form

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

General description

Atto 488 Maleimide is a hydrophilic fluorescent label with high molecular absorption (90.000) and quantum yield (0.80). The fluorescence activity is excited at a maximum of 501nm, and the maximum emission is 523nm. Atto 488 Maleimide is optimized for argon laser excitation and characterized by high photostability. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Post coupling to a substrate, the label carries a net electrical charge of -1.

Application

Atto 488 Maleimide is used for labelling amines, which usually requires a higher pH than the reaction of maleimides with thiols. It is a well-suited fluorescent label for thiol groups.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
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    BCCL9502
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