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18470

Sigma-Aldrich

Bromothymol Blue sodium salt

for microscopy (Bot., Hist., Vit.), indicator (pH 6.0-7.6)

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Synonym(s):
Bromthymol Blue sodium salt, 3′,3′′-dibromothymolsulfonephthalein sodium salt
Empirical Formula (Hill Notation):
C27H27Br2NaO5S
CAS Number:
34722-90-2
Molecular Weight:
646.36
Beilstein:
6887926
EC Number:
252-169-7
MDL number:
MFCD00077263
UNSPSC Code:
12352200
PubChem Substance ID:
57647742
NACRES:
NA.25

grade

for microscopy (Bot., Hist., Vit.)
for molecular biology
indicator (pH 6.0-7.6)

Quality Level

100

form

powder

technique(s)

titration: suitable

visual transition interval

6.0-7.6, yellow to blue

storage temp.

room temp

SMILES string

[Na+].CC(C)c1cc(c(C)c(Br)c1O)C(=C2\C=C(C(C)C)C(=O)C(Br)=C2C)\c3ccccc3S([O-])(=O)=O

InChI

1S/C27H28Br2O5S.Na/c1-13(2)18-11-20(15(5)24(28)26(18)30)23(17-9-7-8-10-22(17)35(32,33)34)21-12-19(14(3)4)27(31)25(29)16(21)6;/h7-14,30H,1-6H3,(H,32,33,34);/q;+1/p-1/b23-21+;

InChI key

NMKFVGALBGZKGW-FKWCIMQXSA-M

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General description

Bromothymol blue is a pH indicator for weak acids and bases. In neutral solution, it appears bluish-green. At pH above 7.6 it appears blue, and at pH below 6.0 it appears yellow.
Bromothymol blue (BTB) is an anionic dye. It can be protonated or deprotonated to generate yellow or blue. BTB helps to make ion-pair complex, that is used to identify several pharmaceutical compounds with the help of extractive spectrophotometric methods.

Application

Suitable for use:
  • In preparations of pH indicator solutions
  • In phospholipid staining on tissues and cells prepared on microscopy slides

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Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Modified bromthymol blue stain for chromatographic detection of phospholipids.
D F Yarmosh et al.
Clinical chemistry, 23(3), 614-615 (1977-03-01)
Dan Wang et al.
Methods in molecular biology (Clifton, N.J.), 851, 9-46 (2012-02-22)
Pain is a complex sensory experience for which the molecular mechanisms are yet to be fully elucidated. Individual differences in pain sensitivity are mediated by a complex network of multiple gene polymorphisms, physiological and psychological processes, and environmental factors. Here
Determination of Fluoroquinolones in Pharmaceutical Formulations by Extractive Spectrophotometric Methods Using Ion-Pair Complex Formation with Bromothymol Blue
Nguyen T D, et al.
Journal of Analytical Methods in Chemistry, 2018 (2018)

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