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Merck
CN

18470

Bromothymol Blue sodium salt

suitable for microscopy

Synonym(s):

Bromthymol Blue sodium salt, 3′,3′′-dibromothymolsulfonephthalein sodium salt

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About This Item

Empirical Formula (Hill Notation):
C27H27Br2NaO5S
CAS Number:
34722-90-2
Molecular Weight:
646.36
UNSPSC Code:
12171500
NACRES:
NA.25
PubChem Substance ID:
57647742
EC Number:
252-169-7
Beilstein/REAXYS Number:
6887926
MDL number:
MFCD00077263

Key Documents

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Product Name

Bromothymol Blue sodium salt, suitable for microscopy

InChI key

NMKFVGALBGZKGW-FKWCIMQXSA-M

InChI

1S/C27H28Br2O5S.Na/c1-13(2)18-11-20(15(5)24(28)26(18)30)23(17-9-7-8-10-22(17)35(32,33)34)21-12-19(14(3)4)27(31)25(29)16(21)6;/h7-14,30H,1-6H3,(H,32,33,34);/q;+1/p-1/b23-21+;

SMILES string

[Na+].CC(C)c1cc(c(C)c(Br)c1O)C(=C2\C=C(C(C)C)C(=O)C(Br)=C2C)\c3ccccc3S([O-])(=O)=O

grade

Molecular Biology

form

powder

technique(s)

titration: suitable

visual transition interval

6.0-7.6, yellow to blue

suitability

suitable for microscopy

storage temp.

room temp

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Application

Suitable for use:
  • In preparations of pH indicator solutions
  • In phospholipid staining on tissues and cells prepared on microscopy slides

General description

Bromothymol blue is a pH indicator for weak acids and bases. In neutral solution, it appears bluish-green. At pH above 7.6 it appears blue, and at pH below 6.0 it appears yellow.
Bromothymol blue (BTB) is an anionic dye. It can be protonated or deprotonated to generate yellow or blue. BTB helps to make ion-pair complex, that is used to identify several pharmaceutical compounds with the help of extractive spectrophotometric methods.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCH3156
BCCG4427
BCCD4799
BCCC1285
BCBZ9654

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Dan Wang et al.
Methods in molecular biology (Clifton, N.J.), 851, 9-46 (2012-02-22)
Pain is a complex sensory experience for which the molecular mechanisms are yet to be fully elucidated. Individual differences in pain sensitivity are mediated by a complex network of multiple gene polymorphisms, physiological and psychological processes, and environmental factors. Here
Determination of Fluoroquinolones in Pharmaceutical Formulations by Extractive Spectrophotometric Methods Using Ion-Pair Complex Formation with Bromothymol Blue
Nguyen T D, et al.
Journal of Analytical Methods in Chemistry, 2018 (2018)
Modified bromthymol blue stain for chromatographic detection of phospholipids.
D F Yarmosh et al.
Clinical chemistry, 23(3), 614-615 (1977-03-01)
Haixia Chi et al.
Scientific reports, 10(1), 19581-19581 (2020-11-13)
Zinc tetraphenylporphyrin (Zn-TPP) solubilized by GroEL protein cage was prepared as a supramolecular mimic of carbonic anhydrase (CA) for CO2 capture. It is shown that the soluble Zn-TPP-GroEL complex can be formed easily by detergent dialysis. The Zn-TPP/GroEL binding ratio

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