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U1250

Sigma-Aldrich

Urea

ReagentPlus®, ≥99.5%, pellets

Synonym(s):

Carbamide, Carbonyldiamide

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96 TESTS
CN¥3,652.98

CN¥3,652.98

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96 TESTS
CN¥3,652.98

About This Item

Linear Formula:
NH2CONH2
CAS Number:
57-13-6
Molecular Weight:
60.06
Beilstein:
635724
EC Number:
200-315-5
MDL number:
MFCD00008022
UNSPSC Code:
12352100
PubChem Substance ID:
57654718
NACRES:
NA.21

CN¥3,652.98

Please contact Customer Service for Availability
Request a Bulk Order

Quality Level

300

product line

ReagentPlus®

Assay

≥99.5%

form

pellets

mp

132-135 °C (lit.)

solubility

H2O: 8 M

density

1.335 g/mL at 25 °C (lit.)

functional group

amine

SMILES string

NC(N)=O

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

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Show Differences

1 of 4

This Item
SRP3084SRP3327SRP3320
IL-1RA murine recombinant, expressed in E. coli, ≥98% (SDS-PAGE)

SRP6006

IL-1RA murine

IL-1Ra human recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

SRP3084

IL-1Ra human

IL-1 RECEPTOR ANTAGONIST human Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

SRP3327

IL-1 RECEPTOR ANTAGONIST human

IL-36RA human recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

SRP3320

IL-36RA human

assay

≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

biological source

mouse

biological source

human

biological source

human

biological source

human

mol wt

17.4 kDa

mol wt

17.2 kDa

mol wt

17.2 kDa

mol wt

17.0 kDa

form

lyophilized

form

lyophilized

form

lyophilized

form

lyophilized

General description

Urea is a chaotropic agent and is used for protein denaturation. It disturbs the hydrogen bonds in the secondary, tertiary and quaternary structure of proteins. Urea can also disturb hydrogen bonds present in DNA secondary structure.[1]

Application

Urea has been used as a protein denaturing agent.[2][3]
Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Legal Information

ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000418590
    0000418590
    0000418589
    0000418589
    0000418591

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    Find documentation for the products that you have recently purchased in the Document Library.

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    Sheehan D.
    Physical Biochemistry: Principles and Applications (2013)
    Protein and cell wall polysaccharide carbonyl determination by a neutral pH 2, 4-dinitrophenylhydrazine-based photometric assay.
    Georgiou C D, et al.
    Redox Biology, 17, 128-142 (2018)
    Protein carbonyl determination by a rhodamine B hydrazide-based fluorometric assay.
    Georgiou C D, et al.
    Redox Biology, 17, 236-245 (2018)
    Christos D Georgiou et al.
    Redox biology, 17, 236-245 (2018-05-05)
    A new fluorometric assay is presented for the ultrasensitive quantification of total protein carbonyls, and is based on their specific reaction with rhodamine B hydrazide (RBH), and the production of a protein carbonyl-RBH hydrazone the fluorescence of which (at ex/em
    Christos D Georgiou et al.
    Redox biology, 17, 128-142 (2018-04-24)
    A new 2,4-dinitrophenylhydrazine (DNPH)-based photometric assay is developed for the quantification of carbonyls in protein samples from any biological source by protein carbonyl-DNPH hydrazone formation at acidic pH in the presence of denaturing urea, and subsequent hydrazone solubilization in the
    View All Related Papers

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