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Key Documents

51457

Sigma-Aldrich

Urea solution

BioUltra, ~8 M in H2O

Synonym(s):

Carbamide, Carbonyldiamide

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About This Item

Linear Formula:
NH2CONH2
CAS Number:
Molecular Weight:
60.06
Beilstein:
635724
MDL number:
UNSPSC Code:
12352111
PubChem Substance ID:
NACRES:
NA.25

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product line

BioUltra

Quality Level

concentration

~8 M in H2O

impurities

insoluble matter, passes filter test
≤0.005% formaldehyde (HCHO)
≤0.05% biuret

refractive index

n20/D 1.40

pH

8.0-10.0 (25 °C)

anion traces

chloride (Cl-): ≤5 mg/kg
sulfate (SO42-): ≤10 mg/kg

cation traces

Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Na: ≤20 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.03
λ: 280 nm Amax: 0.02

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This Item
333033330033301
material

white PTFE valve

material

white PTFE valve

material

white PTFE valve

material

white PTFE valve

compatibility

for use with 20 mm crimp seal

compatibility

for use with 20/400 mm thread

compatibility

for use with 13/425 mm thread

compatibility

for use with 15/425 mm thread

packaging

pkg of 12 ea

packaging

pkg of 12 ea

packaging

pkg of 12 ea

packaging

pkg of 12 ea

Application

Urea solution has been used as a urea buffer for the denaturation of heavy chain and β2m inclusion body preparations.[1] It has also been used as a component of 1x wash buffer to purify HIS-tagged microphthalmia-associated transcription factor (MITF).[2]

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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    SARS-CoV-2-specific memory T cells will likely prove critical for long-term immune protection against COVID-19. Here, we systematically mapped the functional and phenotypic landscape of SARS-CoV-2-specific T cell responses in unexposed individuals, exposed family members, and individuals with acute or convalescent COVID-19. Acute-phase
    Pakavarin Louphrasitthiphol et al.
    Molecular cell, 79(3), 472-487 (2020-06-13)
    It is widely assumed that decreasing transcription factor DNA-binding affinity reduces transcription initiation by diminishing occupancy of sequence-specific regulatory elements. However, in vivo transcription factors find their binding sites while confronted with a large excess of low-affinity degenerate motifs. Here, using
    L Li et al.
    Cell death and differentiation, 22(7), 1081-1093 (2014-12-20)
    P53 is critically important in preventing oncogenesis but its role in inflammation in general and in the function of inflammatory macrophages in particular is not clear. Here, we show that bone marrow-derived macrophages exhibit endogenous p53 activity, which is increased
    Gurudayal et al.
    Nano letters, 15(6), 3833-3839 (2015-05-06)
    Photoelectrochemical water splitting half reactions on semiconducting photoelectrodes have received much attention but efficient overall water splitting driven by a single photoelectrode has remained elusive due to stringent electronic and thermodynamic property requirements. Utilizing a tandem configuration wherein the total
    Florin Sasarman et al.
    Human molecular genetics, 24(10), 2841-2847 (2015-02-06)
    Addition of the trinucleotide cytosine/cytosine/adenine (CCA) to the 3' end of transfer RNAs (tRNAs) is essential for translation and is catalyzed by the enzyme TRNT1 (tRNA nucleotidyl transferase), which functions in both the cytoplasm and mitochondria. Exome sequencing revealed TRNT1

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