SRP3084
IL-1Ra human
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
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Synonym(s):
ICIL-1RA, IL-1RN, IRAP
UNSPSC Code:
12352200
NACRES:
NA.32
Recommended Products
biological source
human
recombinant
expressed in E. coli
Assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
potency
20-40 ng/mL
mol wt
17.2 kDa
packaging
pkg of 100 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white to off-white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... IL1R1(3554)
General description
The gene IL-1RA (interleukin-1 receptor antagonist) is mapped to human chromosome 2q. It belongs to the IL-1 (interleukin 1) family of proteins. The encoded protein has a TIR (Toll/IL-1 receptor) domain. IL-1RA is produced by corneal epithelial cells, monocytes, neutrophils, macrophages and fibroblasts. It is a naturally occurring inflammatory inhibitor protein. Recombinant human IL-1RA is a 17.2kDa protein consisting of 153 amino acid residues.
Biochem/physiol Actions
Interleukin-1 receptor antagonist (IL-1RA) inhibits the activity of IL-1α (interleukin 1 α) and IL-1β (interleukin 1 β) by competitively blocking binding to their type I and type II receptors. Therapeutically, IL-1RA may help in the treatment of sepsis, rheumatoid arthritis, chronic myelogenous leukemia, asthma, psoriasis, and inflammatory bowel disease. Low production of IL-1RA is observed in HIV-1 (human immunodeficiency virus 1)-infected dendritic cells.
Sequence
MRPSGRKSSK MQAFRIWDVN QKTFYLRNNQ LVAGYLQGPN VNLEEKIDVV PIEPHALFLG IHGGKMCLSC VKSGDETRLQ LEAVNITDLS ENRKQDKRFA FIRSDSGPTT SFESAACPGW FLCTAMEADQ PVSLTNMPDE GVMVTKFYFQ EDE
Physical form
Lyophilized from 5mM Tris, pH 8.0 + 25 mM NaCl.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Recombinant interleukin-1 receptor antagonist (IL-1ra): effective therapy against gram-negative sepsis in rats.
Alexander HR
Surgery, 112, 188-193 (1992)
Interleukin-1 beta, interleukin-1 receptor antagonist and interleukin-6 plasma levels and cytokine gene polymorphisms in chronic lymphocytic leukemia: correlation with prognostic parameters.
Hulkkonen J
Haematologica, 85, 600-606 (2000)
IL-1B and IL-1Ra gene polymorphisms and disease severity in rheumatoid arthritis: interaction with their plasma levels.
Buchs N
Genes and Immunity, 2, 222-228 (2001)
Interleukin-1 receptor antagonist (IL-1RA) prevents apoptosis in ex vivo expansion of human limbal epithelial cells cultivated on human amniotic membrane.
Sun CC
Stem Cells, 24, 2130-2139 (2006)
Colonic explant production of IL-1and its receptor antagonist is imbalanced in inflammatory bowel disease (IBD).
Dionne S
Clinical and Experimental Immunology, 112, 435-442 (1998)
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