U4883
Urea
8 M (after reconstitution with 16 mL high purity water)
Synonym(s):
Urea solution, Carbamide, Carbonyldiamide
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About This Item
Linear Formula:
NH2CONH2
CAS Number:
Molecular Weight:
60.06
Beilstein:
635724
EC Number:
MDL number:
UNSPSC Code:
12352111
PubChem Substance ID:
NACRES:
NA.25
form
powder
Quality Level
concentration
8 M (after reconstitution with 16 mL high purity water)
solubility
H2O: soluble (1 vial plus 16 mL)
SMILES string
NC(N)=O
InChI
1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
InChI key
XSQUKJJJFZCRTK-UHFFFAOYSA-N
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General description
Urea is a chaotropic agent and is used for protein denaturation. It disturbs the hydrogen bonds in the secondary, tertiary and quaternary structure of proteins. It can also disturb hydrogen bonding present in DNA secondary structure.
Application
Useful for solubilizing and denaturing proteins in preparation for 2D-PAGE, enzymatic digests, IMAC Chromatography, and related procedures.
Urea has been used for protein sample preparation for reverse-phase nanospray liquid chromatography-tandem mass spectrometry.
Urea has been used for protein sample preparation for reverse-phase nanospray liquid chromatography-tandem mass spectrometry.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Sheehan D
Physical Biochemistry: Principles and Applications (2009)
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Molecular and Cellular Biology, 28, 5014-5026 (2008)
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P53 is critically important in preventing oncogenesis but its role in inflammation in general and in the function of inflammatory macrophages in particular is not clear. Here, we show that bone marrow-derived macrophages exhibit endogenous p53 activity, which is increased
A mechanically and electrically self-healing supercapacitor.
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Immortalized hepatocyte cell lines show only a weak resemblance to primary hepatocytes in terms of gene expression and function, limiting their value in predicting drug-induced liver injury (DILI). Furthermore, primary hepatocytes cultured on two-dimensional tissue culture plastic surfaces rapidly dedifferentiate
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