Recommended Products
biological source
rat
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
3F10, monoclonal
form
lyophilized
packaging
pkg of 50 μg (11867423001)
pkg of 500 μg (11867431001)
manufacturer/tradename
Roche
isotype
IgG1
epitope sequence
YPYDVPDYA
storage temp.
2-8°C
Related Categories
General description
Anti-HA High Affinity is a monoclonal antibody to the HA-peptide (clone 3F10). Anti-HA High Affinity recognizes the HA peptide sequence (YPYDVPDYA), derived from the influenza hemagglutinin protein. The antibody recognizes its antigenic determinant even when the HA peptide epitope is introduced into unrelated recombinant proteins by a technique known as "epitope tagging".
Specificity
Anti-HA, High Affinity recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).
Immunogen
Amino acids 98-106 from the human influenza virus hemagglutinin protein
Application
Use Anti-HA High Affinity for the detection of native influenza hemagglutinin protein and recombinant proteins that contain the HA epitope using:
- Dot blots
- ELISA
- Immunocytochemistry
- Immunoprecipitation
- Western blots
Quality
Function tested in western blot.
Preparation Note
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
The following concentrations should be taken as a guideline:
- ELISA: for detection 100 ng/ml; for coating 1 to 5 μg/ml
- Immunoprecipitation: 0.5 to 5 μg/ml
- Western blot: 50 to 200 ng/ml
Reconstitution
Add 0.5 ml (11 867 423 001; 50 μg) or 2.5 ml (11 867 431 001; 500 μg) double-distilled water to a final concentration of 100 μg/ml or 200 μg/ml respectively.
Rehydrate for 10 min prior to use.
Rehydrate for 10 min prior to use.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Signal Word
Warning-Warning
Hazard Statements
Hazard Classifications
Aquatic Chronic 3 - Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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