12158167001
Roche
Anti-HA-Biotin, High Affinity (3F10)
from rat IgG1
Synonym(s):
antibody
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About This Item
UNSPSC Code:
12352203
biological source
rat
Quality Level
conjugate
biotin conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
3F10, monoclonal
form
lyophilized (stabilized)
packaging
pkg of 50 μg
manufacturer/tradename
Roche
isotype
IgG1
epitope sequence
YPYDVPDYA
storage temp.
2-8°C
Related Categories
General description
Anti-HA-Biotin, High Affinity (3F10) is a monoclonal antibody for the highly sensitive detection of HA-tagged recombinant proteins, Fab fragments, conjugated to biotin. The Anti-HA-Biotin, High Affinity antibody (clone 3F10) recognizes the same epitope as clone 12CA5. It is a monoclonal antibody whose high affinity and low working concentrations result in less cross-reactivity than with other antibodies to the HA-epitope. Anti-HA-Biotin, High Affinity (3F10) is a biotin conjugate of this clone which is specifically useful in western blotting, ELISA applications and assays using the universal biotin-streptavidin platform, by allowing specific and highly sensitive detection of HA-tagged proteins.
Specificity
Anti-HA-Biotin, High Affinity (3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).
Immunogen
Amino acids 98-106 from the human influenza virus hemagglutinin protein
Application
Anti-HA-Biotin, High Affinity (3F10) is used for the detection of HA-tagged recombinant proteins using:
It has also been used for immunocytochemistry, immunofluorescence and αScreen format based assay.
- Dot blots
- ELISA (enzyme-linked immunosorbent assay)
- Western blots
It has also been used for immunocytochemistry, immunofluorescence and αScreen format based assay.
Quality
Function test: The Anti-HA-Biotin; High Affinity is function tested by Western blot analysis of a HA-tagged fusion protein.
Preparation Note
Sample Materials
Sample preparation: Prepare protein extracts containing the HA-tagged protein of interest using any of a variety of standard methods. The following lysis buffers have performed well and should be taken as guidelines:
Sample preparation: Prepare protein extracts containing the HA-tagged protein of interest using any of a variety of standard methods. The following lysis buffers have performed well and should be taken as guidelines:
- Bacterial extracts: 20 mM Tris, pH 8.0, 100 mM NaCl, cOmplete Protease Inhibitor Cocktail Tablets, followed by freeze-thaw.
- Mammalian extracts: 50 mM Tris, pH 7.5, 150 mM NaCl, 0.1% Nonidet P40, complete Protease Inhibitor Cocktail Tablets.
- Other cell lysis buffers may be more appropriate for individual applications. In general, to obtain optimal performance of the affinity matrix:
- Use protease inhibitors to reduce proteolytic activity. Use complete Protease Inhibitor Cocktail Tablets for most applications.
- Limit detergent to the lowest concentration levels necessary to obtain adequate cell lysis.
Working concentration: Working concentration of conjugate depends on application and substrate
The following concentrations should be taken as a guideline:
The following concentrations should be taken as a guideline:
- Dot blot: 100 ng/ml
- ELISA: 100 ng/ml
- Western blot: 100 ng/ml
Reconstitution
Add 1 ml double-distilled water to a final concentration of 50 μg/ml.
Rehydrate for 10 minutes prior to use.
Rehydrate for 10 minutes prior to use.
Other Notes
For life science research only. Not for use in diagnostic procedures.
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Skin Sens. 1
WGK
WGK 2
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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