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DIUTP-RO

Roche

Digoxigenin-11-dUTP, alkali-labile

=85% (HPLC), solution, pkg of 25 μL (11573152910 [1 mM]), pkg of 125 μL (11573179910 [1 mM]]])

Synonym(s):

Digoxigenin-11-dUTP, alkali-labile, digoxigenin

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1 EA
CN¥6,780.43

CN¥6,780.43

Available to ship onApril 27, 2025Details

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1 EA
CN¥6,780.43

About This Item

UNSPSC Code:
41105500

CN¥6,780.43

Available to ship onApril 27, 2025Details

Quality Level

100

Assay

85% (HPLC)

form

solution

packaging

pkg of 125 μL (11573179910 [1 mM]]])
pkg of 25 μL (11573152910 [1 mM])

manufacturer/tradename

Roche

storage temp.

−20°C

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Fmoc-Phe(SO3Na)-OH Novabiochem®

852378

Fmoc-Phe(SO3Na)-OH

Fmoc-(FmocHmb)Phe-OH Novabiochem®

852081

Fmoc-(FmocHmb)Phe-OH

Fmoc-Phe(CF2PO3)-OH Novabiochem®

852288

Fmoc-Phe(CF2PO3)-OH

Fmoc-Tyr(SO3nP)-OH Novabiochem®

852347

Fmoc-Tyr(SO3nP)-OH

form

powder

form

powder

form

powder

form

powder

functional group

sulfonic acid

functional group

Fmoc

functional group

Fmoc

functional group

sulfonic acid

product line

Novabiochem®

product line

Novabiochem®

product line

Novabiochem®

product line

Novabiochem®

storage temp.

15-25°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-10 to -25°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

reaction suitability

reaction type: Fmoc solid-phase peptide synthesis

reaction suitability

reaction type: Fmoc solid-phase peptide synthesis

reaction suitability

reaction type: Fmoc solid-phase peptide synthesis

reaction suitability

reaction type: Fmoc solid-phase peptide synthesis

General description

Digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP), alkali-labile is provided as in 1 mM tetralithium salt, solution. In which DIG is bound to dUTP via an alkali-sensitive ester linkage.

Application

Digoxigenin-11-dUTP, alkali-labile can be used for non-radioactive DNA labeling e.g. random primed or nick translation. It can also be used for labeling probes which are preferred in hybridization experiments where stripping and reprobing of the membrane is intended.

Biochem/physiol Actions

Digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP) replaces deoxythymidine triphophate (dTTP) in the random-primed DNA labeling reaction or in nick translation in a ratio of 35% DIG-11-dUTP and 65% dTTP. It is ideal as a substrate for DNA polymerase, Taq DNA polymerase, terminal transferase and reverse transcriptase.

Other Notes

For life science research only. Not for use in diagnostic procedures.
When this nucleotide is subjected to 0.1 - 0.5 NaOH at +15 to +25°C, the DIG moiety will be removed. It is therefore ideal for use in DIG-labeled probes that can be removed by alkali denaturation (e.g ., in stripping and reprobing of blots). However, if you intend to make a DIG-labeled DNA that will survive alkaline treatment, do not use this alkali-labile preparation. Instead, use the alkali-stable form of DIG-11-dUTP.

also commonly purchased with this product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

does not flash

Flash Point(C)

does not flash

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    10739659
    10739653
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    10739648
    10739647

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