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Safety Information

11207750910

Roche

Anti-Digoxigenin-Rhodamine, Fab fragments

from sheep

Synonym(s):

anti-digoxigenin, digoxigenin

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1 PKG
CN¥5,586.77

CN¥5,586.77

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1 PKG
CN¥5,586.77

About This Item

UNSPSC Code:
12352203

CN¥5,586.77

Please contact Customer Service for Availability

biological source

sheep

Quality Level

100

conjugate

rhodamine conjugate

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized (clear, red solution after reconstitution)

packaging

pkg of 200 μg

manufacturer/tradename

Roche

isotype

IgG

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particle size

5 μm

particle size

3.5 μm

particle size

5 μm

particle size

5 μm

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

product line

Kromasil®

product line

Kromasil®

product line

Kromasil®

product line

Kromasil®

agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

General description

Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems.[1] This product contains Fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to rhodamine.

Specificity

Polyclonal antibodies from sheep show 100% reactivity with digoxigenin and digoxin, but no cross-reactivity with other steroids, such as human estrogens (e.g., estradiol) or androgens (e.g., testosterone).

Application

Use Anti-Digoxigenin-Rhodamine, Fab fragments for the detection of digoxigenin-labeled compounds using:
  • Digoxigenin-labeled sugars in glycoconjugate research
  • Fluorescent in situ hybridization (FISH)[2][3][4]
  • Immunohistocytochemistry[5]
  • In situ hybridization[6]
  • DNA fiber assay[7][8]

Preparation Note

After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer.

Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline: Detection of digoxigenin-labeled sugars in glycoconjugates: 20 to 50 μg/ml
  • Fluorescent in situ hybridization (FISH): 1 to 20 μg/ml
  • Immunohistocytochemistry: 20 to 50 μg/ml
  • In situ hybridization: 20 to 50 μg/ml

Working solution: PBS, 0.5% bovine serum albumin (w/v), pH 7.4.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding. Furthermore pH can be increased up to pH 8.5 to 9.0.

Storage conditions (working solution): Always prepare fresh!

Reconstitution

Add 1 ml double-distilled water to a final concentration of 200 μg/ml.

Other Notes

For life science research only. Not for use in diagnostic procedures.

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Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317,H412

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Regulatory Information

含少量动物源组分生物产品
常规特殊物品
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    Certificates of Analysis (COA)

    Lot/Batch Number
    67161600
    24410900
    63662100
    51720500
    35710000

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    Camila A Quercia et al.
    Comparative cytogenetics, 14(1), 61-74 (2020-02-12)
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    Galina Pendinen et al.
    Genome, 51(9), 714-720 (2008-09-06)
    Thirty-six percent of the wild potato (Solanum L. section Petota Dumort.) species are polyploid, and about half of the polyploids are tetraploid species (2n = 4x = 48). Determination of the type of polyploidy and development of the genome concept
    Spencer W Luebben et al.
    Nucleic acids research, 41(22), 10283-10297 (2013-09-06)
    HELQ is a superfamily 2 DNA helicase found in archaea and metazoans. It has been implicated in processing stalled replication forks and in repairing DNA double-strand breaks and inter-strand crosslinks. Though previous studies have suggested the possibility that HELQ is
    Maximilian H Fitz-James et al.
    eLife, 9 (2020-09-12)
    During mitosis chromosomes reorganise into highly compact, rod-shaped forms, thought to consist of consecutive chromatin loops around a central protein scaffold. Condensin complexes are involved in chromatin compaction, but the contribution of other chromatin proteins, DNA sequence and histone modifications
    R Symonová et al.
    Cytogenetic and genome research, 141(2-3), 153-162 (2013-09-21)
    We applied comparative genomic hybridization (CGH) and genomic in situ hybridization (GISH) to examine genomes of artificially produced sturgeon hybrids between sterlet, Acipenser ruthenus female (∼120 chromosomes) or Russian sturgeon, A. gueldenstaedtii female (∼240 chromosomes) and a spontaneous triploid Siberian
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    Articles

    Digoxigenin (DIG) Labeling Methods

    Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

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