D8156
Monoclonal Anti-Digoxin antibody produced in mouse
clone DI-22, ascites fluid
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Synonym(s):
Monoclonal Anti-Digoxin
UNSPSC Code:
12352203
NACRES:
NA.43
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
DI-22, monoclonal
contains
15 mM sodium azide
technique(s)
dot blot: suitable
flow cytometry: suitable
indirect ELISA: 1:10,000 using digoxin-BSA
indirect ELISA: 1:2,500 using digoxigenin-transferrin
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
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General description
Monoclonal Anti-Digoxin (mouse IgG1 isotype) is derived from the hybridoma DI-22 produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with digoxin- keyhole limpet hemocyanin (KLH). Digoxin is obatained from the leaves of the Digitalis lanata, a foxglove plant. The digoxin molecule is made up of a sugar and a cardenolide. It has a molecular weight is 780.95 Da. It is a odorless white crystal, which is soluble in alcohol and freely soluble in pyridine. It is expressed in the duodenum, kidneys, liver and the blood-brain barrier.
Specificity
Antibody is specific for digoxin and shows a high affinity for digoxigenin.
Monoclonal Anti-Digoxin antibody is specific for digoxin and digoxin-labeled compounds, and shows strong cross-reactivity with digoxigenin.
Immunogen
Digoxin-KLH.
Application
Monoclonal Anti-Digoxin antibody produced in mouse has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- dot blot
- flow cytometry
- fluorescence in situ hybridization (FISH)
- DNA hybridization
- in-situ hybridization (ISH)
- immunodetection of the probe and chromosome X
Biochem/physiol Actions
Digoxin is a phytoestrogen that binds estrogen receptors and produces inotropic effects in cardiac muscle. Studies show that digoxin decreases the risk of breast and uterus cancers that are estrogen sensitive.
Digoxin is a substrate for P-glycoprotein (P-gp), a membrane efflux transporter. This antibody may be used to detect digoxin-labeled compounds such as oligonucleotides, antibodies or peptides.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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Effect of hormones on the concentration of a digoxin-like material in Tetrahymena
Csaba G, et al.
Acta Protozoologica, 44, 81-84 (2005)
Drug Metabolism in Cardiovascular Disease
Drug Metabolism in Diseases, 139-156 (2017)
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Journal of virology, 88(7), 3861-3873 (2014-01-24)
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Waqar Ahmed et al.
Scientific reports, 8(1), 15438-15438 (2018-10-20)
Epstein-Barr virus-encoded RNAs (EBER1 and EBER2) are two highly abundant, non-protein coding RNAs consistently expressed in all EBV infected cells, but their function remains poorly understood. Conventional in situ hybridization studies have indicated that these RNAs are present exclusively in
GSTT1-dependent induction of centromere-negative and-positive micronuclei by l, 2: 3, 4-diepoxybutane in cultured human lymphocytes
Vlachodimitropoulos D, et al.
Mutagenesis, 12(5), 397-403 (1997)
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