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Merck
CN

11333089001

Roche

Anti-Digoxigenin

from sheep

Synonym(s):

anti-digoxigenin, digoxigenin

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.54

Key Documents

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Product Name

Anti-Digoxigenin, from sheep

biological source

sheep

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized

packaging

pkg of 200 μg

manufacturer/tradename

Roche

isotype

IgG

storage temp.

2-8°C

Quality Level

100

Related Categories

Analysis Note

No cross reactivity with other steroids, such as human estrogens e.g., estradiol or androgens e.g., testosterone.

Application

Anti-Digoxigenin has been used in DNA tethering. It has been used to attach DNA molecule to the glass surface of the flow cell.
Use Anti-Digoxigenin antibody for the detection of digoxigenin-labeled compounds using:
  • ELISA
  • Immunohistocytochemistry
  • In situ hybridization
  • Western blot

Biochem/physiol Actions

In the presence of Na+, Mg2+ and ATP, digoxigenin inhibits sodium pumps.
The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.

General description

Digoxigenin is a hapten which is used in labeling of nucleic acids and in detection systems.
Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes. It allows faster detection, is less hazardous and has an increased shelf life.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline:
  • ELISA: for coating: 2 to 4 μg/ml
  • Immunohistocytochemistry: 0.5 to 2 μg/ml
  • In situ hybridization: 0.2 to 0.4 μg/ml
  • Western blot: 0.5 to 2 μg/ml

Working solution: For coating applications: PBS (phosphate buffered saline), pH 7.4
Add 1 ml PBS to a final concentration of 200 μg/ml.
After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption.

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pictograms

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GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

does not flash

flash_point_c

does not flash

Regulatory Information

常规特殊物品
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Certificates of Analysis (COA)

Lot/Batch Number
66890900
66085700
51081900
47120700
28401200

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Methods in Molecular Biology (2011)
Affinity Labeling of a Sulfhydryl Group in the Cardiacglycoside Receptor Site of Na+/K+?ATPase by N?Hydroxysuccinimidyl Derivatives of Digoxigenin.
Antolovic R, et al.
European Journal of Biochemistry, 227(1?2), 61-67 (1995)
Jonathan Silver et al.
Biosensors & bioelectronics, 63, 117-123 (2014-07-30)
We describe a proof-of-principle, immune sandwich assay in which immune complexes link micron-size beads via DNA tethers to a sensor surface. The number of tethered beads, counted using low-magnification microscopy, provides a measure of the concentration of analyte. The prototype
Nonradioactive labeling of probe with digoxigenin by polymerase chain reaction.
Lion T and Haas O A
Analytical Biochemistry, 188(2), 335-337 (1990)
Electron paramagnetic resonance of a single NV nanodiamond attached to an individual biomolecule.
Teeling-Smith R M, et al.
Biophysical Journal, 110(9), 2044-2052 (2016)
View All Related Papers

Articles

Digoxigenin (DIG) Labeling Methods

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

地高辛 (DIG) 标记方法

地高辛（DIG）标记方法和试剂盒适用于DNA和RNA DIG探针、随机引物DNA标记、切口平移标记、5'和3'寡核苷酸末端标记。

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