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2GRHSRMKB
Roche
KAPA2G Robust HotStart ReadyMix
2 × ( contains 2 mM MgCl2 at 1×)
Synonym(s):
PCR, hotstart PCR
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100 ML
CN¥571.58
1 L
CN¥804.93
2 L
CN¥1,270.48
18 L
CN¥8,299.78
CN¥571.58
Available to ship onApril 10, 2025Details
All Photos(5)
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100 ML
CN¥571.58
1 L
CN¥804.93
2 L
CN¥1,270.48
18 L
CN¥8,299.78
About This Item
UNSPSC Code:
41106300
NACRES:
NA.55
CN¥571.58
Available to ship onApril 10, 2025Details
Recommended Products
usage
sufficient for 100 reactions
sufficient for 500 reactions
Quality Level
shelf life
≤12 mo.
feature
Difficult Templates/Specialty Enzymes PCR
dNTPs included
hotstart
packaging
pkg of 1.25 mL (100 x 25 μL rxn; KK5701)
pkg of 6.25 mL (500 x 25 μL rxn; KK5702)
manufacturer/tradename
Roche
concentration
2 × ( contains 2 mM MgCl2 at 1×)
technique(s)
PCR: suitable
input
crude DNA
storage temp.
−20°C
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This Item | 179337 | V800262 | 179957 |
|---|---|---|---|
| assay 99.8% | assay ≥99.8% | assay ≥99.5% | assay ≥99.6% |
| grade anhydrous | grade ACS reagent | grade anhydrous | grade Laboratory Reagent |
| Quality Level 200 | Quality Level 100, 200 | Quality Level - | Quality Level 100 |
| form liquid | form liquid | form liquid | form liquid |
| density 0.791 g/mL at 25 °C (lit.) | density 0.791 g/mL at 25 °C (lit.) | density 0.791 g/mL at 25 °C (lit.) | density 0.791 g/mL at 25 °C (lit.) |
General description
The second-generation KAPA2G Robust DNA Polymerase was evolved to solve inconsistent amplification across a broad range of amplicon types (GC- and AT-rich). KAPA2G Robust HotStart® ReadyMix™ enables higher processivity and specific activity, which translates to robust PCR performance, high sensitivity, and improved tolerance to common inhibitors. The high performance of the KAPA2G Robust DNA Polymerase is ideally suited for challenging PCR applications and difficult samples, eliminating the need for optimization using multiple enzymes and protocols.
Application
KAPA2G Robust HotStart® ReadyMix™ has been used for the:
- amplification of GC- or AT-rich templates
- aplification of templates containing common polymerase chain reaction (PCR) inhibitors e.g. salts, urea, SDS, or ethanol
- crude sample PCR e.g. buccal swabs, cultured mammalian, yeast, or bacterial cells
- colony PCR[1]
- DNA extraction and amplification[2][3]
- mutation screening[4]
- methylation-specific PCR (MSP) amplification[5]
- genotyping-PCR[6]
- PCR-based sequencing[7]
Biochem/physiol Actions
DNA fragments generated with KAPA2G Robust HotStart® DNA Polymerase have the same characteristics as DNA fragments generated with wild-type Taq DNA polymerase and may be used for routine downstream analyses or applications, including restriction enzyme digestion, cloning, and sequencing. Like wild-type Taq, KAPA2G Robust HotStart has 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′→5′ exonuclease (proofreading) activity. The fidelity of KAPA2G Robust HotStart is similar to that of wild-type Taq; it has an error rate of approximately 1 error per 1.7 x 105 nucleotides incorporated. PCR products generated by KAPA2G Robust HotStart are 3′-dA-tailed and may be cloned into TA cloning vectors.
Features and Benefits
Efficient amplification of GC- and AT-rich targets
Improved tolerance to common PCR inhibitors
Unrivalled performance in colony PCR
Quick Notes:
- Robust performance across a wide range of GC- and AT-rich templates
- Increased PCR success rates
Improved tolerance to common PCR inhibitors
- Efficient amplification from crude samples
- Higher yield and sensitivity per unit of enzyme
Unrivalled performance in colony PCR
- Higher yields and improved consistency of PCR direct from E. coli and yeast cells
Quick Notes:
- KAPA2G Robust HotStart ReadyMix PCR Kits contain KAPA2G Robust DNA Polymerase, engineered for high processivity and inhibitor tolerance.
- Both purified genomic DNA and crude samples (e.g. colony PCR) can be used as template.
- Use 15 sec/kb extension time per cycle, and increase to 30-60 sec/kb for difficult amplicons or templates.
- KAPA2G Robust HotStart ReadyMix contains 2 mM MgCl2 at 1X.
- KAPA2G Robust HotStart ReadyMix with dye includes two inert tracking dyes to allow direct loading onto agarose gels.
- KAPA2G Robust HotStart ReadyMix is ideal for single-protocol PCR, i.e. amplification of fragments varying from 25–65% GC, up to 1 kb in size, using a single reaction setup and cycling protocol.
- Reaction products are 3′-dA-tailed and may be cloned into TA cloning vectors.
Quality
Each batch of KAPA2G Robust HotStart DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA2G Robust HotStart ReadyMix PCR Kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.
Preparation Note
Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage. Provided that all components have been handled carefully and not contaminated, the kit is not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days). Long-term storage at room temperature and 4°C is not recommended. Please note that reagents stored at temperatures above -20°C are more prone to degradation when contaminated during use, and therefore storage at such temperatures is at the user′s own risk.
Other Notes
For Research Use Only. Not for use in diagnostic procedures.
Legal Information
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
Kit Components Only
Product No.
Description
- KAPA2G Robust HotStart® DNA Polymerase 1U per 25 µL reaction
- MgCl2 2mM at 1X
- dNTP Mix 0.2 mM of each dNTP at 1X
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - STOT SE 2
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Regulatory Information
含少量动物源组分生物产品
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