Recommended Products
usage
sufficient for 1000 reactions
sufficient for 500 reactions
sufficient for 5000 reactions
Quality Level
shelf life
≤18 mo.
feature
dNTPs included: no
hotstart
packaging
pkg of 250 U (KK1508)
pkg of 2500 U (KK1513)
pkg of 500 U (KK1510)
manufacturer/tradename
Roche
technique(s)
PCR: suitable
input
purified DNA
storage temp.
−20°C
Looking for similar products? Visit Product Comparison Guide
Application
KAPA Taq HotStart® has been used for:
- High throughput PCR
- Amplification of low copy DNA templates
- Multiplex PCR
- Specific amplification of complex templates
- RT-PCR
- confronting two-pair primers-polymerase chain reaction (CTPP-PCR)
- end-point PCR
Biochem/physiol Actions
KAPA Taq DNA Polymerase is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. KAPA Taq and KAPA Taq HotStart® DNA Polymerase have 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′ → 5′ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 105 nucleotides incorporated. In the HotStart formulation, the KAPA Taq is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity.
Features and Benefits
High performance
Quick Notes:
- Improved sensitivity, specificity, and yields.
- Novel buffer formulation facilitates specific primer annealing, leading to higher yield of specific product.
Quick Notes:
- KAPA Taq HotStart® DNA Polymerase can replace any commercial hotstart Taq DNA polymerase in an existing protocol. The final MgCl2 concentration and annealing temperature may need to be optimized to account for differences in formulation.
- The KAPA Taq HotStart Buffer is a uniquelyformulated buffer offering improved specificity and sensitivity, and improved amplification of GC- and AT-rich templates.
- The KAPA Taq HotStart Buffer does not contain MgCl2; MgCl2 (25 mM) is supplied separately to allow greater flexibility during reaction setup.
- The KAPA Taq HotStart PCR Kit is suitable for the amplification of fragments up to 3.5 kb from genomic DNA or 5 kb from less complex targets.
Quality
Each batch of KAPA Taq HotStart® DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA Taq HotStart PCR kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.
Preparation Note
Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long term storage.
Other Notes
For Research Use Only. Not for use in diagnostic procedures.
Legal Information
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
Kit Components Only
Product No.
Description
- KAPA Taq Standard or HotStart® DNA Polymerase 5 U/μL
- 10X KAPA Taq Buffer A
- 10X KAPA Taq Buffer B
- 10X KAPA Buffer with loading dye (optional)
- 5X KAPA Taq HotStart® Buffer (HotStart kits only)
- MgCl2 25 mM
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - STOT SE 2
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
The VNTR 48 bp polymorphism in the DRD4 gene is associated with higher tobacco smoking in male Mexican mestizo smokers with and without COPD
Perez-Rubio G, et al.
Diagnostics (Basel, Switzerland), 10 (2020)
Confronting two biomolecular techniques to detect NRF2 gene polymorphism biomarkers
Chiarella P, et al.
Future science OA, 5 (2018)
Functional characterisation of a Fads2 fatty acyl desaturase with Δ6/Δ8 activity and an Elovl5 with C16, C18 and C20 elongase activity in the anadromous teleost meagre (Argyrosomus regius).
Monroig O, et al.
Aquaculture (Amsterdam, Netherlands), 412, 14-22 (2013)
Articles
An overview of directed evolution and the methods for generating proteins with optimized or entirely new functions.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service