TXHSRMKB
Roche
KAPA Taq EXtra HotStart ReadyMix
suitable for PCR
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Synonym(s):
KAPA, PCR, hotstart PCR, taq DNA polymerase
UNSPSC Code:
41106300
NACRES:
NA.55
Recommended Products
feature
dNTPs included
hotstart
Quality Level
packaging
pkg of 1.25 mL (KK3604)
pkg of 6.25 mL (KK3605)
manufacturer/tradename
Roche
technique(s)
PCR: suitable
input
purified DNA
storage temp.
−20°C
General description
KAPA Taq EXtra HotStart® ReadyMix with dye (2X) is a ready-to-use cocktail containing all components for short- to long-range PCR, except primers and template. The 2X ReadyMix contains KAPA Taq EXtra HotStart DNA Polymerase (0.675 U per 25 μL reaction), KAPA Taq EXtra Buffer (1X), dNTPs (0.3 mM of each dNTP at 1X), MgCl2 (2 mM at 1X) and stabilizers. The 2X ReadyMix also contains two inert tracking dyes to enable direct loading of PCR products onto agarose gels for analysis by electrophoresis, without the need to add a DNA loading solution.
Application
KAPA Taq EXtra HotStart® ReadyMix™ has been used for:
- PCR of amplicons to be analyzed by agarose gel electrophoresis
- PCR of short and medium length targets (<5 kb)
- Long-range PCR (5 – 15 kb)
- PCR with limiting amounts of template DNA
Features and Benefits
- KAPA Taq EXtra HotStart® is a blend of KAPA Taq HotStart and a proofreading DNA polymerase
- Suitable for short-, medium- and long-range PCR, and PCR with low amounts of template DNA
- Fidelity is 2–4 times better than Taq
- For short amplicons, replace Taq DNA polymerase for improvements in yield and/or fidelity.
- Amplify mid- to long-range targets with high yield and sensitivity.
- 2X ReadyMix with dye contains two inert tracking dyes to allow loading of PCR products directly onto agarose gels for analysis.
Quality
Each batch of KAPA Taq EXtra HotStart® DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). The 2X KAPA Taq EXtra HotStart ReadyMix is subjected to stringent quality control tests, is free of contaminating exo- and endonuclease activity, and meets strict requirements with respect to DNA contamination levels.
Preparation Note
Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage.
Other Notes
For Research Use Only. Not for use in diagnostic procedures.
Legal Information
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
Kit Components Only
Product No.
Description
- KAPA Taq EXtra HotStart® DNA Polymerase 0.675U per 25μL reaction
- KAPA Taq EXtra Buffer (1X)
- 1X dNTPs 0.3 mM of each dNTP
- 1X MgCl2 2 mM
- Stabilizers
- Two inert tracking dyes
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Regulatory Information
常规特殊物品
含少量动物源组分生物产品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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