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Roche

KAPA Taq EXtra HotStart ReadyMix

suitable for PCR

Synonym(s):

KAPA, PCR, hotstart PCR, taq DNA polymerase

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Select a Size

25 ML
¥1,035.55
100 ML
¥3,340.09

¥1,035.55

Please contact Customer Service for Availability
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25 ML
¥1,035.55
100 ML
¥3,340.09

About This Item

UNSPSC Code:
41106300
NACRES:
NA.55

¥1,035.55

Please contact Customer Service for Availability

feature

dNTPs included
hotstart

Quality Level

100

packaging

pkg of 1.25 mL (KK3604)
pkg of 6.25 mL (KK3605)

manufacturer/tradename

Roche

technique(s)

PCR: suitable

input

purified DNA

storage temp.

−20°C

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sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

feature

closure type Tape-seal

feature

closure type Zipper-lock

feature

closure type Zipper-lock

feature

closure type Tape-seal

W × L × H

51 in. × 58 in. × 25 nL, average inflated dimensions

W × L × H

23 in. × 38 in. × 11 in., average inflated dimensions

W × L × H

45 in. × 58 in. × 25 in., average inflated dimensions

W × L × H

39 in. × 48 in. × 15 in., inflated dimensions

W × L

51 in. × 58 in. , deflated dimensions

W × L

23 in. × 43 in. , deflated dimensions

W × L

48 in. × 60 in. , deflated dimensions

W × L

38 in. × 51.5 in. , average deflated dimensions

gas volume

520 L

gas volume

1.5 ft3

gas volume

500 L

gas volume

280 L

General description

KAPA Taq EXtra HotStart® ReadyMix with dye (2X) is a ready-to-use cocktail containing all components for short- to long-range PCR, except primers and template. The 2X ReadyMix contains KAPA Taq EXtra HotStart DNA Polymerase (0.675 U per 25 μL reaction), KAPA Taq EXtra Buffer (1X), dNTPs (0.3 mM of each dNTP at 1X), MgCl2 (2 mM at 1X) and stabilizers. The 2X ReadyMix also contains two inert tracking dyes to enable direct loading of PCR products onto agarose gels for analysis by electrophoresis, without the need to add a DNA loading solution.

Application

KAPA Taq EXtra HotStart® ReadyMix™ has been used for:
  • PCR of amplicons to be analyzed by agarose gel electrophoresis
  • PCR of short and medium length targets (<5 kb)
  • Long-range PCR (5 – 15 kb)
  • PCR with limiting amounts of template DNA

Features and Benefits

  • KAPA Taq EXtra HotStart® is a blend of KAPA Taq HotStart and a proofreading DNA polymerase
  • Suitable for short-, medium- and long-range PCR, and PCR with low amounts of template DNA
  • Fidelity is 2–4 times better than Taq
  • For short amplicons, replace Taq DNA polymerase for improvements in yield and/or fidelity.
  • Amplify mid- to long-range targets with high yield and sensitivity.
  • 2X ReadyMix with dye contains two inert tracking dyes to allow loading of PCR products directly onto agarose gels for analysis.

Quality

Each batch of KAPA Taq EXtra HotStart® DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). The 2X KAPA Taq EXtra HotStart ReadyMix is subjected to stringent quality control tests, is free of contaminating exo- and endonuclease activity, and meets strict requirements with respect to DNA contamination levels.

Preparation Note

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Legal Information

HOTSTART is a registered trademark of Molecular BioProducts, Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

Product No.
Description
  • KAPA Taq EXtra HotStart® DNA Polymerase 0.675U per 25μL reaction
  • KAPA Taq EXtra Buffer (1X)
  • 1X dNTPs 0.3 mM of each dNTP
  • 1X MgCl2 2 mM
  • Stabilizers
  • Two inert tracking dyes

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Regulatory Information

常规特殊物品
含少量动物源组分生物产品

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Certificates of Analysis (COA)

Lot/Batch Number
99472
96779
96407
86439
86148

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