2GFHSRMDKB
Roche
KAPA2G Fast HotStart ReadyMix
2 ×, with dye
Sign Into View Organizational & Contract Pricing
All Photos(4)
Synonym(s):
PCR
UNSPSC Code:
41106300
NACRES:
NA.55
Recommended Products
Quality Level
usage
sufficient for 100 reactions
sufficient for 500 reactions
shelf life
≤18 mo.
feature
Fast PCR
Multiplex PCR
dNTPs included
hotstart
packaging
kit of 1.25 mL (100 x 25 μL rxn; KK5608)
kit of 6.25 mL (500 x 25 μL rxn; KK5609)
manufacturer/tradename
Roche
concentration
2 ×
technique(s)
PCR: suitable
input
purified DNA
storage temp.
−20°C
Looking for similar products? Visit Product Comparison Guide
General description
KAPA2G Fast DNA Polymerase is a second-generation (2G) enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase. In addition to speed, KAPA2G Fast provides higher yields and sensitivity than competitor enzymes across a broad range of targets.
KAPA2G Fast DNA Polymerase Kits are designed for fast PCR, in which total reaction times are 20–70% shorter than those of conventional PCR assays performed with wild-type Taq DNA polymerase. This can be achieved without sacrificing reaction performance, and does not require specialized PCR consumables or thermocyclers.
KAPA2G Fast HotStart® DNA Polymerase is supplied in a convenient 2X ReadyMix format and is devoid of primers and template. The ReadyMix comprises KAPA2G Fast HotStart DNA Polymerase (0.5 U per 25 μL reaction) in a proprietary reaction buffer containing dNTPs (0.2 mM of each dNTP at 1X), MgCl2 (1.5 mM at 1X) along with stabilizers.
KAPA2G Fast DNA Polymerase Kits are designed for fast PCR, in which total reaction times are 20–70% shorter than those of conventional PCR assays performed with wild-type Taq DNA polymerase. This can be achieved without sacrificing reaction performance, and does not require specialized PCR consumables or thermocyclers.
KAPA2G Fast HotStart® DNA Polymerase is supplied in a convenient 2X ReadyMix format and is devoid of primers and template. The ReadyMix comprises KAPA2G Fast HotStart DNA Polymerase (0.5 U per 25 μL reaction) in a proprietary reaction buffer containing dNTPs (0.2 mM of each dNTP at 1X), MgCl2 (1.5 mM at 1X) along with stabilizers.
Application
KAPA2G Fast HotStart® ReadyMix™ has been used in:
- Routine PCR
- Fast PCR
- Genotyping
- Multiplex allele-specific colony-PCR (MASC-PCR)
- Genotyping polymerase chain reaction (PCR)
Biochem/physiol Actions
KAPA2G Fast HotStart® DNA Polymerase generated DNA may be used for routine downstream analyses or applications, including restriction enzyme digestion, cloning and sequencing. Like wild-type Taq, KAPA2G Fast has 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′→5′ exonuclease (proofreading) activity. The fidelity of KAPA2G Fast is similar to that of wild-type Taq; it has an error rate of approximately 1 error per 1.7 x 105 nucleotides incorporated.
Features and Benefits
Reduce cycling times up to 75% :
High speed, higher performance :
Quick Notes :
- Extension times as low as 1 sec per kilobase
- Broad coverage of both AT- and GC-rich targets
High speed, higher performance :
- Single copy sensitivity from complex targets
- Increased yields
Quick Notes :
- KAPA2G Fast HotStart ReadyMix PCR Kits contain the engineered KAPA2G Fast HotStart DNA Polymerase, developed for fast PCR.
- Use 1 sec extension time for amplicons <1 kb and 15 sec/kb for longer amplicons, and save 20–70% of total reaction time.
- No need for specialized instrumentation or PCR consumables.
- Optimized buffer system offers improved yields, specificity and sensitivity, facilitating efficient primer annealing across a wide range of primer lengths, GC contents, and melting temperatures.
- For high reaction efficiency, do not exceed 25 μL reaction volumes.
- KAPA2G Fast HotStart ReadyMix contains 1.5 mM MgCl2 at 1X.
- KAPA2G Fast HotStart ReadyMix with dye includes two inert tracking dyes to allow direct loading onto agarose gels.
- Reaction products are 3′-dA-tailed and may be cloned into TA cloning vectors.
Quality
Each batch of KAPA2G Fast DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA2G Fast ReadyMix is subjected to stringent quality control tests, is free of contaminating exo- and endonuclease activity, and meets strict requirements with respect to DNA contamination levels.
Preparation Note
Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage. Provided that all components have been handled carefully and not contaminated, the kit is not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days). Long-term storage at room temperature and 4°C is not recommended. Please note that reagents stored at temperatures above -20°C are more prone to degradation when contaminated during use, and therefore storage at such temperatures is at the user′s own risk.
Other Notes
For Research Use Only. Not for use in diagnostic procedures.
Legal Information
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
STOT SE 2
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Regulatory Information
新产品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Precise manipulation of bacterial chromosomes by conjugative assembly genome engineering.
Ma N J, et al.
Nature Protocols, 9(10), 2285-2285 (2014)
Alvaro Villarreal-Ponce et al.
Cell reports, 33(8), 108417-108417 (2020-11-26)
Unveiling the molecular mechanisms underlying tissue regeneration provides new opportunities to develop treatments for diabetic ulcers and other chronic skin lesions. Here, we show that Ccl2 secretion by epidermal keratinocytes is directly orchestrated by Nrf2, a prominent transcriptional regulator of
Articles
An overview of directed evolution and the methods for generating proteins with optimized or entirely new functions.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service