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form
liquid
manufacturer/tradename
Novagen®
storage condition
do not freeze
application(s)
life science and biopharma
storage temp.
2-8°C
General description
His•Bind Resin is an uncharged IDA agarose resin. The binding capacity is approximately 8 mg/ml bed volume. His•Bind Resin is well suited to small to medium scale purifications using either gravity flow columns or batch methods.
Application
His•Bind® Resin have been used: in purification of His•Tag® fusion proteins by metal chelation chromatography, for purification of the bacterially produced OCRL1WT/VAR phosphatase domain to study heterogeneity in Lowe Syndromein protein purification to study anaerobic phloroglucinol degradation by Clostridium scatologenes for isolation of His6-tagged proteins for studying the role of human repressor activator protein 1 (RAP1) and glial fibrillary acidic protein (GFAPɛ)proteins in increasing γ-secretase activity in a yeast model system.
Biochem/physiol Actions
His•Bind® resin helps in the purification of proteins containing the His-tagged sequence by the process of metal chelation chromatography. It utilizes the charge buffer provided in the His•Bind® Buffer Kit, that binds the His-tagged sequence effectively with Ni2+ or Co2+ cations that are immobilized onto the His•Bind® resin. The target protein can be easily recovered by eluting with imidazole. The His•Bind® Resin is designed to be regenerated and reused multiple times, making it a cost-effective solution. Its versatility allows for protein purification under gentle, non-denaturing conditions, or in the presence of either guanidine or urea, depending on the specific requirements of the purification process.
Features and Benefits
- Reusable many times
- Compatible with His•Bind® Buffer Kit
- Compatible with 1 mM THP
Warning
Toxicity: Standard Handling (A)
Legal Information
HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Flam. Liq. 3
WGK
WGK 1
Flash Point(F)
86.0 °F
Flash Point(C)
30 °C
Regulatory Information
危险化学品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Anaerobic phloroglucinol degradation by Clostridium scatologenes
Zhou Y, et al.
Environmental Microbiology, 14(4) (2023)
Heterogeneity in Lowe Syndrome: Mutations Affecting the Phosphatase Domain of OCRL1 Differ in Impact on Enzymatic Activity and Severity of Cellular Phenotypes
J Lee J, et al.
Biomolecules, 13(4), 615-615 (2023)
The human RAP1 and GFAP? proteins increase ?-secretase activity in a yeast model system
Swanson MJ, et al.
G3: Genes, Genomes, Genetics, 13(8) (2023)
Articles
This article shows the use of BugBuster® and Benzonase® as protein purification tools to extract recombinant proteins from E. coli and to reduce the viscosity of the extract.
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