1.46073
HEIMPLATE XLD Agar - LI EP+USP 30ml
Selective media for the isolation of Salmonella and Shigella in pharmaceuticals and clinical materials
Synonym(s):
Xylose Lysine Deoxycholate Agar
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About This Item
Agency
EP 2.6.12
USP 61
Quality Level
sterility
sterile
form
solid
feature
ready-to-use
technique(s)
microbiological culture: suitable
pH
7.4 ( in H2O)
application(s)
microbiology
pharmaceutical
storage temp.
20-25°C
suitability
Salmonella spp. (spp.)
Related Categories
General description
Xylose Lysine Deoxycholate agar (XLD agar) is a selective growth medium introduced by Taylor (1965) for the isolation of Salmonella and Shigella species (Taylor and schelbert, 1971). It relies on the use of selective inhibitory component sodium deoxycholate and three indicator systems, i.e. xylose, lactose and sucrose combined with phenol red. Degradation of xylose, lactose, and sucrose to acid causes phenol red to change its colour to yellow. Sodium thiosulfate and iron (III) salt react to form a precipitate of black iron sulfide in the colonies, indicating the production of hydrogen sulfide. Bacteria which decarboxylate lysine to cadaverine can be recognized by the appearance of a purple coloration around the colonies due to an increase in pH. These reactions can proceed simultaneously or successively. This causes the pH indicator to exhibit various shades of colour, or it may change its colour from yellow to red on prolonged incubation. The culture medium is weakly inhibitory to other microbes due to the presence of sodium deoxycholate.
Application
Xylose Lysine Deoxycholate (XLD) Agar - LI is a selective culture medium for isolation of Salmonella and Shigella in pharmaceuticals.
Packaging
90 mm settle plates with 30 ml filling volume (20 or 120 plates per box) for long incubation (LI)
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 2
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Handbook of culture media for food and water microbiology
Royal Society of Chemistry (2011)
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