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Key Documents

Safety Information

OGS590

Sigma-Aldrich

PUC19 - HIGH COPY BLUE/WHITE CLONING PLASMID

plasmid vector for molecular cloning

Synonym(s):

cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector

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100 μG
CN¥4,472.02

CN¥4,472.02

Available to ship onApril 14, 2025Details

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100 μG
CN¥4,472.02

About This Item

UNSPSC Code:
12352200
NACRES:
NA.85

CN¥4,472.02

Available to ship onApril 14, 2025Details

form

buffered aqueous solution

mol wt

size 2686 bp

bacteria selection

ampicillin

Origin of replication

pUC (500 copies)

Peptide cleavage

no cleavage

Promoter

Promoter name: Lac
Promoter activity: inducible
Promoter type: bacterial

reporter gene

none

shipped in

ambient

storage temp.

−20°C

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This Item
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PUC19 - HIGH COPY BLUE/WHITE CLONING PLASMID plasmid vector for molecular cloning

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PUC19 - HIGH COPY BLUE/WHITE CLONING PLASMID

PSF-CMV-PUC18 - CMV PUC18 MCS PLASMID plasmid vector for molecular cloning

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PSF-CMV-PUC18 - CMV PUC18 MCS PLASMID

PSF-CMV-PUC19 - CMV PUC19 MCS PLASMID plasmid vector for molecular cloning

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PSF-CMV-PUC19 - CMV PUC19 MCS PLASMID

PSF-CAG-UB-HYGRO - CAG HYGROMYCIN PLASMID plasmid vector for molecular cloning

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PSF-CAG-UB-HYGRO - CAG HYGROMYCIN PLASMID

peptide cleavage

no cleavage

peptide cleavage

no cleavage

peptide cleavage

no cleavage

peptide cleavage

no cleavage

origin of replication

pUC (500 copies)

origin of replication

pUC (500 copies)

origin of replication

pUC (500 copies)

origin of replication

pUC (500 copies)

bacteria selection

ampicillin

bacteria selection

ampicillin

bacteria selection

ampicillin

bacteria selection

kanamycin

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

mol wt

size 2686 bp

mol wt

size 4310 bp

mol wt

size 4309 bp

mol wt

size 7694 bp

General description

This high-copy number plasmid is the versatile industry standard cloning vector that can be used to insert and sub-clone genes using blue/white screening.

Application

Cloning in a gene: PUC19 - HIGH COPY BLUE/WHITE CLONING PLASMID is a standard cloning plasmid used by thousands of laboratories for routine cloning. The plasmid can be used for blue-white screening and contains a diverse multiple cloning site that extends from the EcoRI to Hind3 restriction sites that allows DNA sequences to be inserted.

Sequence

To view sequence information for this product, please visit the product page

Analysis Note

To view the Certificate of Analysis for this product, please visit www.oxgene.com

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Storage Class Code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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    Jin-Gyoung Jung et al.
    PLoS genetics, 10(10), e1004751-e1004751 (2014-10-31)
    The Notch3 signaling pathway is thought to play a critical role in cancer development, as evidenced by the Notch3 amplification and rearrangement observed in human cancers. However, the molecular mechanism by which Notch3 signaling contributes to tumorigenesis is largely unknown.
    Geoffrey M Lynn et al.
    Nature biotechnology, 33(11), 1201-1210 (2015-10-27)
    The efficacy of vaccine adjuvants such as Toll-like receptor agonists (TLRa) can be improved through formulation and delivery approaches. Here, we attached small molecule TLR-7/8a to polymer scaffolds (polymer-TLR-7/8a) and evaluated how different physicochemical properties of the TLR-7/8a and polymer
    Diana Romero et al.
    Carcinogenesis, 37(1), 18-29 (2015-10-28)
    Dickkopf-3 (Dkk-3) is a secreted protein whose expression is downregulated in many types of cancer. Endogenous Dkk-3 is required for formation of acini in 3D cultures of prostate epithelial cells, where it inhibits transforming growth factor (TGF)-β/Smad signaling. Here, we
    Alexander C Cerny et al.
    PLoS genetics, 11(10), e1005578-e1005578 (2015-10-29)
    Recycling of signaling proteins is a common phenomenon in diverse signaling pathways. In photoreceptors of Drosophila, light absorption by rhodopsin triggers a phospholipase Cβ-mediated opening of the ion channels transient receptor potential (TRP) and TRP-like (TRPL) and generates the visual

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